Quantitative Expression of Adhesion Molecules on Granulocyte Colony-Stimulating Factor-Mobilized Peripheral Blood,Bone Marrow,and Cord Blood CD34 + Cells

Abstract

The purpose of this study is to investigate the function of the main adhesion receptors on CD34⁺ cells during hematopoietic stem cell transplantation. Expression was quantified by flow cytometry using calibration beads. CD34⁺ cells were isolated from either bone marrow (BM), cord blood (CB), or peripheral blood (PB) from study patients and a control group after granulocyte colony-stimulating factor (G-CSF) administration. The study of the CD34⁺ cell differentiation showed that CD34⁺ cells are mainly CD38⁺ and HLA-DR⁺, whatever the type of harvest. However, quantitative analysis elicited a weaker expression of CD38 on PB and CB CD34⁺ cells in comparison to BM CD34⁺ cells. The proportions of CD34⁺/CD49d⁺ and CD34⁺/CD49e⁺ were smaller on PB cells, without quantitative expression variation. This phenotypic variation promotes CD34⁺ cells to exit from BM into circulation, inducing the mobilization. The homing of the CD34⁺ cells to the BM involves the CD62L receptor. The expression of this receptor was found to be more frequent and stronger on PB cells than on BM or CB cells. The CD11b, CD18, and CD54 receptors are implicated in CD34⁺ cell adhesion to BM microenvironment. No significant variation in CD34⁺/CD11b⁺ and CD34⁺/CD18⁺ cell frequency was noted. Moreover, the CD54 receptor was more frequently expressed on CB and PB cells. Quantitative analysis revealed that CD18 was more strongly expressed on BM than on PB cells to promote progenitors adhesion by interacting with stromal cells. Finally, the quantitative expression of the main receptors on CD34⁺ cells explained cellular functions during the different steps of hematopoietic stem cells transplantation.