Is Helium Insufflation Superior to Carbon Dioxide Insufflation in Bacteremia and Bacterial Translocation with Peritonitis?

Abstract

Purpose

: To evaluate the effects of CO₂ or helium insufflation on bacteremia and bacterial translocation in rats with peritonitis.

Materials and Methods

: Forty male Wistar-Albino rats were divided into four groups, each containing 10 rats. The rats in the first group were injected only with E. coli into their peritoneal cavities with no further manipulation. The second group, following E. coli injection, underwent midline laparotomy without manipulation of the viscera for 1 hour. After the injection of E. coli in the third and fourth groups, CO₂ and helium pneumoperitoneum, respectively, were maintained for 1 hour under 14 mm Hg pressure. At the end of the sixth hour, tissue samples were taken from the liver, spleen, lung, and mesenteric lymph nodes in order to evaluate bacterial translocation. During the study, blood samples were taken from each rat at 0, 1, 2, 4, and 6 hours to demonstrate bacteremia.

Results

: There was a significant increase in bacteremia in the CO₂ pneumoperitoneum group compared with the laparotomy-only and helium groups at 1 and 2 hours. Although all the blood samples at the fourth hour were positive for E. coli in every rat of all groups, helium was associated with a lower incidence of bacteremia at the sixth hour compared with other groups (P < 0.05). The CO₂ pneumoperitoneum caused bacterial translocation to all organs from which tissue samples were taken. Although there was an insignificant decrease in translocation to the liver, spleen, and lung with helium compared with CO₂ insufflation, helium did not increase bacterial translocation to the spleen compared with laparotomy alone, as did CO₂ (P < 0.05).

Conclusion

: Helium might be an alternative to CO₂ insufflation in patients with peritonitis if these results are confirmed by further experimental and clinical trials.