Chemokine Pattern in Relation to Disease State in Human Immunodeficiency Virus-Infected Children

Abstract

Although beta chemokines can block human immunodeficiency virus (HIV) entry into target cells, their role in HIV disease progression is controversial. To determine the association of RANTES with HIV disease state, we examined constitutive mRNA expression by reverse-transcribed polymerase chain reaction (RT-PCR) in peripheral blood mononuclear cells (PBMCs) and induction of RANTES secretion by enzyme-linked immunosorbent assay (ELISA) in anti-CD3 monoclonal antibody (MAb)-stimulated cultures of PBMCs, and in CD4 + and CD8 + T cell subsets of 17 HIV-infected children. In comparison with uninfected subjects, PBMCs of HIV-infected children were deficient in both constitutive RANTES mRNA expression as well as in stimulus-induced RANTES production. Children in clinical category C were found to be more deficient than children in clinical category A. Expression of RANTES mRNA in PMBCs was inversely correlated with plasma virus load and correlated directly with CD4 + T cell counts. In T cell subsets, RANTES production was equivalent between CD4 + and CD8 + T cells in patients and controls but CD8 + T cells of children in clinical category A produced higher RANTES levels than those of children in clinical category C. The beta -chemokine RANTES may play an important role in slowing clinical disease progression in HIV-infected children.