Effects of Neonatal Testosterone and Progesterone on Open-Field Behaviour in the RAT

Abstract

Rats treated on the day of birth with progesterone (50 üg) or testosterone pro-pionate (200 üg) or the oil injection vehicle alone were tested in the open-field on four consectuve days at 45 days and 85 days of age. Averages across treatments showed that females ambulated more and reared more than males at both ages, that they groomed more than males at 45 days of age, and defaecated less at 85 days of age. Progesterone treatment significantly reduced defaecation in males at 45 days of age, and reduced grooming in both sexes. At 85 days of age progesterone significantly increased activity in females. Testosterone-treated animals of both sexes groomed significantly less than same-sex controls at 45 days of age, whereas at 85 days of age activity scores were significantly reduced only in females although testosterone treated males were less active on 2 test days and more active on 1. Early postnatal treatment with progesterone appeared to feminise male rats, and testosterone to masculinise female rats. Both hormones also altered the behaviour of opposite sexed rats, indicating that male rats may be further masculinised by exogenous testosterone and female rats further feminised by progesterone. Progesterone may have acted as an anti-androgenic agent by blocking gonadal and adrenal androgens in males and adrenal androgens in females.