The subtransus deformation behaviour of Ti–10V–2Fe–3Al alloy was investigated. The flow stress curves are featured with significant flow softening, resulting from the breaking up and globularisation of lamellar α phase. The critical major axis of α phase to cause flow softening is 1 µm. Alpha phase acts as a dislocation barrier in the beginning and suppresses the recrystallisation of beta matrix. Dynamic recovery is the main restoration mode for beta matrix and alpha phase just works as a marker of it. Beta phase is dominated by strong {001} and weak {111} orientations. The Burgers relationship and variant selection are observed between α phase and β matrix in a single grain and overall samples, and the subtransus deformation does not destroy it.