Sage Journals: Discover world-class research

Abstract

OBJECTIVE: This study determines the role of changes in numerical densities of apoptotic myonuclei and satellite cells in age-related remodeling of the thyroarytenoid muscle (TA).

DESIGN: Changes in numerical densities of apoptotic myonuclei and satellite cells were estimated for the entire TA by using stereological techniques.

RESULTS: There was an increase in N_{V apoptotic myonucleus, fiber type} (P < 0.05) and in the percentage of apoptotic myonuclei (P < 0.05) in type 1 but not in type 2 muscle fibers. There was also an increase in N_{V apoptotic satellite cell, fiber type} (P < 0.05) and in the percentage of apoptotic satellite cells (P < 0.05) in type 1 fibers but not in type 2 fibers.

CONCLUSION: Apoptosis may contribute to age-related fiber loss and atrophy in the TA.

SIGNIFICANCE: Therapeutic techniques based on decreasing the frequency of apoptosis may block age-related fiber loss and atrophy in the TA.

Get full access to this article

View all access options for this article.

References

Ward

Colton

McConnell

. Aging of the voice and swallowing Otolaryngol Head Neck Surg 1989;100:283–6

Lexell

. Human aging, muscle mass, and fiber type composition J Gerontol A Biol Sci Med Sci 1995;50A:11–6

Malmgren

Fisher

Bookman

. Age-related changes in muscle fiber types in the human thyroarytenoid muscle: An immunohistochemical and stereological study using confocal laser scanning microscopy Otolaryngol Head Neck Surg 1999;121:441–51

Malmgren

Ringwood

. Aging of the recurrent laryngeal nerve: An ultrastructural morphometric study In: Fujimura

, editor. Vocal physiology: voice production, mechanisms and function. New York: Raven Press; 1988. p. 159–80

5. Malmgren

Fisher

Brandes

. Age-related increase in muscle fiber “type grouping” in the human thyroarytenoid muscle. Association for Research in Otolaryngology Twentieth Midwinter Meeting, 1997. http://www.aro.org/abstracts/1997/T597.html

Malmgren

Lovice

Kaufman

. Age-related changes in muscle fiber regeneration in the human thyroarytenoid muscle Arch Otolaryngol Head Neck Surg 2000;126:851–6

Mezzogiorno

Coletta

Zani

. Paracrine stimulation of senescent satellite cell proliferation by factors released by muscle or myotubes from young mice Mech Ageing Dev 1993;70:35–44

Malmgren

Fisher

Jones

. Numerical densities of myonuclei and satellite cells in muscle fiber types in the aging human thyroarytenoid muscle: An immunohistochemical and stereological study using confocal laser scanning microscopy Otolaryngol Head Neck Surg 2000;123:377–84

Thompson

. Apoptosis in the pathogenesis and treatment of disease Science 1995;267:1456–62

10.

Allen

Linderman

Roy

. Apoptosis: A mechanism contributing to remodeling of skeletal muscle in response to hindlimb unweighting Am J Physiol 1997;273:C579–87

11.

Gavrieli

Sherman

Ben-Sasson

. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation J Cell Biol 1992;119:493–501

12.

Sterio

. The unbiased estimation of number and sizes of arbitrary particles using the disector J Microsc 1984;134:127–36

13.

Monemi

Thornell

Eriksson

. Diverse changes in fibre type composition of the human lateral pterygoid and digastric muscles during aging J Neurol Sci 1999;171:38–48

14.

Podhorska-Okolov

Sandri

Zanada

. Apoptosis of myofibres and satellite cells: Exercise-induced damage in skeletal muscle of the mouse Neuropathol Appl Neurobiol 1999;24:518–31

15.

Strasser

Tiefenthaler

Steinlechner

. Urinary incontinence in the elderly and age-dependent apoptosis of rhabdosphincter cells [letter] Lancet 1999;354:918–9

16.

Yoshimura

Harii

. A regenerative change during muscle adaptation to denervation in rats J Surg Res 1999;81:139–46

17.

Tews

Goebel

Schneider

. DNA-fragmentation and expression of apoptosis-related proteins in experimentally denervated and reinnervated rat facial muscle Neuropathol Appl Neurobiol 1997;23:141–9

18.

Benbassat

Maki

Unterman

. Circulating levels of insulin-like growth factor (IGF) binding protein- 1 and −3 in aging men: Relationships to insulin, glucose, IGF, and dehydroepiandrosterone sulfate levels and anthropometric measures J Clin Endocrinol Metab 1997;82:1484–91

19.

Mampuru

Chen

Kalenik

. Analysis of events associated with serum deprivation-induced apoptosis in C3H/Sol8 muscle satellite cells Exp Cell Res 1996;226:372–80

20.

Barton-Davis

Shoturma

Musaro

. Viral mediated expression of insulin-like growth factor I blocks the aging-related loss of skeletal muscle function Proc Natl Acad Sci USA 1998;95:15603–7

21.

Shiotani

O'Malley

Bwj

Coleman

. Human insulinlike growth factor 1 gene transfer into paralyzed rat larynx: Single vs multiple injection Arch Otolaryngol Head Neck Surg 1999;125:555–60

22.

Malmgren

. Age-related changes in blood flow rates in the intrinsic laryngeal muscles of young adult and old rats during quiet respiration [abstract] Abstracts of the Fifteenth Midwinter Research Meeting of the Association for Research in Otolaryngology 1992;31

Muscle Fiber and Satellite Cell Apoptosis in the Aging Human Thyroarytenoid Muscle: A Stereological Study with Confocal Laser Scanning Microscopy

Abstract

Get full access to this article

References