Abstract
Departments of *Clinical Chemistry and †Neurosurgery, University Hospital, Uppsala, and ‡Department of Pharmacology, Karolinska Institute, Stockholm, Sweden
(Article appeared in J Cereb Blood Flow Metab 9:607–616 (1989))
Elements from Tables 1, 2, 3, and 4 that appeared on pages 609, 611, 612, and 614 are inadvertently left out. They appear correctly below.
Concentrations (µM) of lactate, pyruvate, and some purine metabolites in microdialysates from the striatum of rats subjected to MCAO
Samples were collected in three 30-min fractions before and six after MCAO (after fraction 3) in five rats and analyzed by HPLC. L, left, occluded side; R, right, control side. Values were means, SEM in parentheses. * Significantly different from control side. IF, an increase factor was calculated for each analyte as the ratio of the total amount of the analyte in fractions 4–6 to the amount in fractions 1–3. Significantly different from fractions 1, 2, and 3. From fractions 2 and 3. From fraction 3.
Fraction number
Metabolite
1
2
3
MCAO
4
5
6
7
8
9
IF
Lactate L
159.8
116.0
110.3
435.7
a
467.8
a
500.7
a
469.5
a
416.2
a
421.2
a
3.6
(32.9)
(27.0)
(29.4)
(68.2)
(52.8)
(68.1)
(71.1)
(70.2)
(111.5)
*
*
*
*
*
*
Lactate R
140.0
110.8
111.4
104.7
129.3
129.6
86.2
84.2
65.0
1.0
(38.3)
(31.4)
(29.1)
(20.7)
(26.4)
(38.8)
(41.2)
(43.7)
(5.5)
Pyruvate L
8.6
7.0
7.7
7.5
9.7
18.2
9.0
9.8
14.8
1.5
(1.3)
(1.5)
(1.9)
(1.0)
(3.5)
(10.3)
(2.9)
(5.0)
(0.0)
Pyruvate R
6.2
6.1
6.8
6.6
7.0
6.5
6.3
6.3
5.0
1.1
(1.0)
(1.0)
(1.4)
(1.4)
(1.1)
(0.7)
(1.3)
(2.7)
(0.0)
Adenosine L
0.1
0.1
0.1
1.9
b
2.7
a
2.1
a
2.0
b
1.1
0.9
22.3
(0.0)
(0.0)
(0.0)
(0.3)
(0.6)
(0.5)
(0.6)
(0.3)
(0.4)
*
*
*
*
Adenosine R
0.1
0.1
0.1
0.1
0.1
0.2
0.1
0.1
0.1
1.3
(0.0)
(0.0)
(0.0)
(0.0)
(0.0)
(0.1)
(0.0)
(0.0)
(0.0)
Inosine L
0.5
0.3
0.3
2.2
6.0
a
4.1
b
5.3
a
4.7
a
5.5
a
11.2
(0.1)
(0.0)
(0.0)
(0.3)
(1.7)
(0.8)
(1.1)
(1.0)
(0.4)
*
*
*
*
*
*
Inosine R
0.4
0.3
0.3
0.3
0.4
0.5
0.3
0.3
0.2
1.2
(0.0)
(0.0)
(0.0)
(0.0)
(0.1)
(0.1)
(0.0)
(0.0)
(0.0)
Hypoxanthine L
1.1
0.4
0.3
3.3
6.1
a
6.3
a
5.4
b
5.6
b
6.8
a
8.7
(0.4)
(0.1)
(0.0)
(0.6)
(1.6)
(1.4)
(1.3)
(1.6)
(1.7)
*
*
*
*
Hypoxanthine R
0.6
0.5
0.4
0.5
0.8
1.1
0.5
0.5
0.4
1.6
(0.1)
(0.1)
(0.0)
(0.1)
(0.2)
(0.5)
(0.1)
(0.1)
(0.0)
Concentrations of amino acids in microdialysates from the striatum of rats subjected to MCAO
Samples were collected in three 30-min fractions before and six after MCAO (after fraction 3) in four rats and analyzed by HPLC. Concentrations were pmol/10 μl. L, left, occluded side; R, right, control side. Values were means, SEM in parentheses, asp, aspartate; glu, glutamate; ser, serine; gin, glutamine; gly, glycine; arg, arginine; thr, threonine; tau, taurine; ala, alanine; tyr, tyrosine. 'Significantly different from control side. IF, increase factor (see Table 1). Significantly different from fractions 1, 2, and 3. From fraction 1.
Fraction number
Amino acid
1
2
3
MCAO
4
5
6
7
8
9
IF
asp L
3.3
1.3
1.3
18.0
a
32.0
a
21.8
a
24.1
a
20.5
a
19.4
a
12.2
(0.7)
(0.2)
(0.2)
(6.1)
(1.9)
(5.6)
(6.0)
(3.5)
(3.1)
*
*
*
*
*
*
asp R
3.1
1.7
1.6
2.0
1.9
1.4
2.5
1.3
2.6
0.7
(1.9)
(0.5)
(0.4)
(0.6)
(0.3)
(0.3)
(1.1)
(0.3)
(1.1)
glu L
5.4
4.2
4.5
79.7
a
192.1
a
154.8
a
204.1
a
221.4
a
210.6
a
30.3
(0.8)
(1.0)
(1.2)
(18.8)
(26.8)
(42.1)
(5.0)
(7.2)
(14.7)
*
*
*
*
*
*
gluR
9.2
8.1
8.0
10.0
11.4
11.3
14.6
14.6
12.9
1.3
(3.5)
(3.0)
(3.1)
(2.3)
(2.2)
(2.5)
(3.9)
(4.1)
(3.1)
ser L
18.1
16.0
15.3
18.8
26.5
31.0
23.4
25.2
26.6
1.5
(2.0)
(2.7)
(2.0)
(3.0)
(5.0)
(10.1)
(6.0)
(3.9)
(3.9)
*
*
ser R
16.7
15.6
13.8
14.4
12.2
9.8
12.6
9.9
11.0
0.8
(2.4)
(2.2)
(1.8)
(2.7)
(2.5)
(0.8)
(1.7)
(0.8)
(2.1)
gln L
254.6
249.6
230.1
145.0
194.8
244.9
223.0
219.5
208.2
0.8
(25.3)
(26.0)
(19.2)
(16.8)
(30.6)
(45.8)
(56.2)
(41.0)
(48.3)
*
*
gln R
253.3
235.4
193.7
167.2
117.9
95.0
b
104.1
b
117.3
b
117.3
b
0.6
(26.0)
(28.3)
(21.1)
(23.4)
(18.4)
(22.9)
(31.0)
(39.3)
(26.1)
gly/arg/thr L
9.2
8.2
8.3
9.2
14.7
21.2
14.9
17.1
18.6
1.8
(0.9)
(1.3)
(1.3)
(1.4)
(3.8)
(8.6)
(4.0)
(2.4)
(2.0)
gly/arg/thr R
9.4
8.4
7.3
7.5
6.2
5.5
6.4
5.4
5.7
0.8
*
*
(1.3)
(0.7)
(0.7)
(0.8)
(0.5)
(0.5)
(1.0)
(0.9)
(1.6)
tau L
20.4
16.2
16.0
312.2
a
452.3
a
496.1
a
528.1
a
478.8
a
426.0
a
24.0
(3.0)
(3.9)
(4.0)
(61.5)
(48.9)
(61.6)
(83.5)
(48.0)
(15.8)
*
*
*
*
*
*
tau R
19.9
16.5
13.9
12.1
17.1
26.1
14.1
14.2
14.5
1.1
(2.2)
(1.8)
(1.4)
(2.1)
(4.1)
(12.9)
(2.4)
(2.8)
(2.8)
ala/tyr L
9.4
7.2
6.9
21.5
40.9
a
52.6
a
51.5
a
57.4
a
59.8
a
4.9
(0.5)
(0.9)
(1.0)
(3.1)
(2.5)
(7.7)
(8.7)
(6.0)
(4.7)
*
*
*
*
*
*
*
ala/tyr R
7.0
6.3
5.6
5.9
5.6
5.4
6.3
5.2
6.1
0.9
(0.4)
(0.3)
(0.3)
(0.7)
(0.7)
(0.4)
(0.8)
(0.4)
(1.0)
Concentrations of amino acids in microdialy sat es from the striatum of rats subjected to MCAO
Samples were collected in three 30-min fractions before and six after MCAO (after fraction 3) in four rats and analyzed by HPLC. Concentrations were pmol/10 μl. L, left, occluded side; R, right, control side. Values were means, SEM in parentheses, met, methionine; try, tryptophan; val, valine; phe, phenylalanine; iso, isoleucine; leu, leucine; lys, lysine. *Significantly different from control side. IF, increase factor (see Table 1). Significantly different from fractions 1, 2, and 3. From fraction 2.
Fraction number
Amino acid
1
2
3
MCAO
4
5
6
7
8
9
IF
GABA L
0.4
0.0
0.0
8.3
36.0
48.8
44.3
70.5
a
74.4
a
233
(0.2)
(0.0)
(0.0)
(3.1)
(16.1)
(16.1)
(9.3)
(17.8)
(23.0)
*
*
*
*
GABA R
0.5
0.1
0.0
0.0
0.2
0.1
0.2
0.3
0.2
0.5
(0.3)
(0.0)
(0.0)
(0.0)
(0.1)
(0.1)
(0.1)
(0.1)
(0.1)
met/try L
2.1
1.7
2.0
2.4
3.3
3.8
4.4
a
5.3
a
5.9
a
1.6
(0.1)
(0.2)
(0.3)
(0.5)
(0.4)
(0.6)
(0.5)
(0.4)
(0.6)
*
*
*
*
*
met/try R
2.0
2.0
2.2
2.5
1.9
1.6
1.8
1.6
1.6
1.0
(0.2)
(0.1)
(0.2)
(0.6)
(0.1)
(0.1)
(0.1)
(0.0)
(0.1)
vai l
6.6
6.0
6.1
6.7
11.1
20.8
a
11.8
13.4
14.6
2.1
(0.9)
(1.1)
(1.1)
(0.7)
(2.8)
(10.7)
(1.8)
(1.2)
(1.3)
*
*
*
val r
6.1
5.8
5.3
5.6
4.3
3.8
4.5
3.8
4.0
0.8
(0.5)
(0.4)
(0.3)
(0.7)
(0.4)
(0.3)
(0.5)
(0.4)
(0.9)
phe 1
2.5
2.3
2.4
3.7
4.8
6.6
8.1
b
9.2
a
9.5
a
2.1
(0.2)
(0.5)
(0.4)
(1.4)
(0.8)
(2.1)
(2.2)
(2.6)
(2.4)
pheR
2.3
2.3
1.9
4.0
2.6
2.3
2.3
2.6
3.2
1.4
(0.3)
(0.3)
(0.0)
(0.8)
(0.4)
(0.5)
(0.6)
(0.8)
(1.0)
iso L
3.6
3.0
3.2
3.6
4.7
5.8b
6.7a
7.1a
7.3a
1.4
(0.1)
(0.5)
(0.2)
(0.3)
(0.2)
(1.0)
(1.0)
(0.8)
(0.7)
*
*
*
*
*
iso R
3.6
3.5
3.2
4.0
2.7
2.0
2.8
2.2
2.4
0.8
(0.3)
(0.2)
(0.4)
(0.8)
(0.2)
(0.2)
(0.3)
(0.2)
(0.6)
leu L
5.9
6.0
5.7
5.7
9.9
18.6
a
11.8
13.3
14.3
1.9
(0.6)
(1.4)
(0.8)
(0.4)
(2.2)
(8.8)
(1.6)
(1.1)
(1.0)
*
*
*
leu R
5.7
5.3
4.8
6.9
3.9
3.3
4.0
3.2
4.4
0.9
(0.4)
(0.3)
(0.4)
(1.9)
(0.3)
(0.0)
(0.5)
(0.6)
(0.7)
lys L
7.6
7.1
7.2
5.0
6.2
10.6
3.2
4.4
4.7
1.0
(2.4)
(3.0)
(3.1)
(2.3)
(3.5)
(7.7)
(0.2)
(1.0)
(1.2)
lys R
7.5
6.5
5.7
6.9
4.2
3.2
3.7
3.6
3.5
0.7
(2.4)
(2.0)
(1.7)
(1.6)
(1.0)
(1.0)
(0.6)
(0.4)
(0.0)
Concentrations of dopamine (DA) and DA metabolites in microdialy sates from the striatum of rats subjected to MCAO
Samples were collected in three 30-min fractions before and six after MCAO (after fraction 3) in five rats and analyzed by HPLC. Concentrations were pmol/20 μl. L, left, occluded side; R, right, control side. Values were means, SEM in parentheses. 5-HIAA = serotonin metabolite that appears in the same chromatograms. * Significantly different from control side. IF, increase factor (see Table 1). Significantly different from fractions 1, 2, and 3. From fraction 3.
Fraction number
Metabolite
1
2
3
MCAO
4
5
6
7
8
9
IF
DAL
0.2
0.1
0.1
16.7
a
7.2
a
4.5
3.9
3.4
2.1
70.3
(0.0)
(0.0)
(0.0)
(3.4)
(1.1)
(0.9)
(0.9)
(0.1)
(0.3)
*
*
*
*
*
*
DAR
0.1
0.1
0.1
0.4
0.5
0.5
0.5
0.2
0.2
5.3
(0.0)
(0.0)
(0.0)
(0.1)
(0.3)
(0.3)
(0.2)
(0.0)
(0.0)
DOPAC L
18.9
19.4
20.4
9.3
b
6.6
a
5.8
a
5.9
a
5.3
a
6.7
a
0.4
(2.7)
(3.7)
(4.0)
(2.0)
(1.4)
(1.8)
(1.5)
(1.5)
(1.7)
*
*
*
*
*
*
DOPAC R
20.9
20.8
19.9
21.2
20.1
18.4
19.8
18.9
20.6
1.0
(3.5)
(3.1)
(3.0)
(2.1)
(1.9)
(2.8)
(1.9)
(4.7)
(2.3)
5-HIAA L
2.4
2.3
2.9
1.6
1.0
1.1
0.9
0.7
1.0
0.5
(0.7)
(1.1)
(1.0)
(0.3)
(0.2)
(0.2)
(0.2)
(0.1)
(0.2)
5-HIAA R
2.0
1.5
2.0
2.1
2.0
1.7
2.3
1.4
2.5
1.1
(0.6)
(0.5)
(0.5)
(0.5)
(0.5)
(0.5)
(0.7)
(0.7)
(0.5)
HVAL
15.3
16.7
16.9
5.8
a
3.9
a
3.9
a
3.7
a
2.4
a
3.8
a
0.3
(2.2)
(2.9)
(3.0)
(1.8)
(1.5)
(1.6)
(1.6)
(0.9)
(1.5)
*
*
*
*
*
*
HVAR
18.3
17.8
17.0
16.8
16.9
17.0
16.7
15.2
17.2
1.0
(2.3)
(1.8)
(1.6)
(1.0)
(1.2)
(1.6)
(1.0)
(5.3)
(1.8)