Objectives: To measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, −2, and −3 during the inflammatory, proliferative, and remodeling phases of rat vocal fold injury.
Study Design: Prospective, animal model.
Subjects and Methods: Vocal folds were injured in 30 rats. Injured specimens were harvested on postinjury days 1, 3, 7, 14, 28, and 56. Five uninjured rats were used for polymerase chain reaction (PCR) control. Real-time PCR was used to measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, −2, and −3.
Results: Compared with control, expression of procollagen-I and -III were significantly decreased on postinjury days 1 and 56; decorin expression was significantly decreased on postinjury days 1, 3, 7, and 56; HAS-1 expression was significantly decreased on postinjury days 3, 7, 14, 28, and 56; HAS-2 expression was significantly decreased on postinjury days 28 and 56; HAS-3 expression was significantly decreased on postinjury day 56.
Conclusions: Results revealed time-dependent alterations in the expression of genes coding procollagen-I and -III, decorin, and HAS-1, −2, and −3. Knowledge of the temporal regulation of these genes and underlying histology will be used in future studies to investigate molecular approaches for manipulation of vocal fold injury.
Get full access to this article
View all access options for this article.
References
1.
. DaileySHFordCN. Surgical management of sulcus vocalis and vocal fold scarring.Otolaryngol Clin North Am2006;39:23–42
2.
. ThibeaultSLGraySDBlessDM. Histologic and rheologic characterization of vocal fold scarring.J Voice2002;16:96–104
3.
. RousseauBHiranoSScheidtTD. Characterization of vocal fold scarring in a canine model.Laryngoscope2003;113:620–7
4.
. ThibeaultSLBlessDMGraySD. Interstitial protein alterations in rabbit vocal fold with scar.J Voice2003;17:377–83
5.
. TateyaTTateyaISohnJH. Histologic characterization of rat vocal fold scarring.Ann Otol Rhinol Laryngol2005;114:183–91
6.
. TateyaTTateyaISohnJH. Histological study of acute vocal fold injury in a rat model.Ann Otol Rhinol Laryngol2006;115:285–92
7.
. LimXTateyaITateyaT. Immediate inflammatory response and scar formation in wounded vocal folds.Ann Otol Rhinol Laryngol2006;115:921–9
8.
. WelhamNVLimXTateyaI. Inflammatory factor profiles one hour following vocal fold injury.Ann Otol Rhinol Laryngol2008;117:145–52
9.
. SongWErgulA. Type-2 diabetes-induced changes in vascular extracellular matrix gene expression: relation to vessel size.Cardiovasc Diabetol2006;17:5–3
10.
. TashiroTHiraokaHIkedaY. Effect of GDF-5 on ligament healing.J Orthop Res2006;24:71–9
11.
. ToedaKNakamuraKHirohataS. Versican is induced in infiltrating monocytes in myocardial infarction.Mol Cell Biochem2005;280:47–56
12.
. OishiYKatoHNoguchiT. Dietary protein as a potent regulator of the hyaluronan synthase gene in rat skin.Biosci Biotechnol Biochem2003;67:736–42
13.
. FujisakiKTanabeNSuzukiN. The effect of IL-1alpha on the expression of matrix metalloproteinases, plasminogen activators, and their inhibitors in osteoblastic ROS 17/2.8 cells.Life Sci2006;78:1975–82
. PeledZMChinGSLiuW. Response to tissue injury.Clin Plast Surg2000;27:489–500
16.
. SayaniKDoddCMNedelecB. Delayed appearance of decorin in healing burn scars.Histopathology2000;36:262–72
17.
. GraySDTitzeIRChanR. Vocal fold proteoglycans and their influence on biomechanics.Laryngoscope1999;109:845–54
18.
. KrishnaPRosenCABranskiRC. Primed fibroblasts and exogenous decorin: potential treatments for subacute vocal fold scar.Otolaryngol Head Neck Surg2006;135:937–45
19.
. WeigelPHFullerGMLeBouefRD. A model for the role of hyaluronic acid and fibrin in the early events during the inflammatory response and wound healing.J Theoret Biol1986;119:219–234
20.
. DufloSThibeaultSLLiW. Effect of a synthetic extracellular matrix on vocal fold lamina propria gene expression in early wound healing.Tissue Eng2006;12:3201–7
