Abstract
Problem
To determine the various types of connexins and their localization within the rat larynx.
Methods
Eight larynges from 3–4 month old Fisher-344 rats were used. Four were immediately dissected to obtain the epiglottis, vocal folds, supraglottic and subglottic mucosa. RNA was extracted and used as a template for production of cDNA, which was used with primers for Cx26, Cx30, Cx32, Cx37, Cx40, and Cx43 and RT-PCR performed. Four others were serially sectioned and the sections processed for immunohistochemistry.
Results
RT-PCR revealed a high level of Cx43, Cx32, and Cx30 within epiglottis and Cx43 in the vocal folds. Immunohistochemical staining confirmed these results.
Conclusion
The rat epiglottis is rich in Cx43, Cx32, and Cx30 while the vocal folds contain Cx43. Their localizations suggest involvement in secretion for protective purposes and they may play a key role in laryngeal pathologies.
Significance
Cxs in the larynx may be disrupted by decreased pH, alcohol ingestion, and smoke inhalation. These three factors contribute significantly to the pathology that patients commonly present to the otolaryngologist. Potential pharmacologic treatments are on the horizon and further characterization of the laryngeal connexins needs to be carried out before such treatments can be utilized.
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