The Impact of a Cation Channel Blocker (Furosemide) on Pseudomonas aeruginosa PA01 Biofilm Architecture

Abstract

OBJECTIVE: To determine if furosemide could have a role in inhibition of biofilm formation.

STUDY DESIGN: A prospective study of 72-hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls.

METHODS: Bacteria were treated with furosemide and controls to determine its antiplanktonic properties. PA01 biofilms were treated with serial 2-fold dilutions of furosemide and controls over a 48-hour period at pH concentrations of 7.0, 8.0, and 9.0.

RESULTS: Furosemide had no effect on planktonic PA01. It had minimal effect at pH below 8.0. A pH of 8.0 and 9.0 reduced biofilms 25%. Furosemide 10 mg/mL reduced biofilms 50% at pH 8.0 and 9.0. It inhibited biofilms to <30% at concentrations of 0.6 to 0.16 mg/mL at pH 8.0 and <10% at concentrations of 1.25 to 0.3 mg/mL at pH 9.0.

CONCLUSION: Furosemide was found to be nonantiplanktonic, but does appear to destabilize preformed PA01 biofilms at pH-dependent concentrations.