Sage Journals: Discover world-class research

Abstract

OBJECTIVE: To measure alterations in SPA1, A2, and D gene expression in various forms of inflammatory chronic rhinosinusitis (CRS).

STUDY DESIGN AND SETTING: Sinus mucosal biopsies were performed in patients with allergic fungal rhinosinusitis (AFS), CRS with nasal polyposis, cystic fibrosis (CF), and controls. SP mRNA was measured with quantitative polymerase chain reaction.

RESULTS: Patients with CF (n = 4) showed significantly increased SPA1 (82-fold), SPA2 (100-fold), and SPD (47-fold) mRNA (P < 0.05) when compared with controls (n = 5). Patients with CRS with nasal polyposis (n = 5) also demonstrated elevated SPA1 (27-fold), SPA2 (13-fold), and SPD (13-fold). Patients with AFS (n = 7) had increased SPA1 (5-fold), SPA2 (9-fold), and SPD (17-fold), but were not statistically significant.

CONCLUSION: SPA1, A2, and D are upregulated in various forms of CRS, but are significantly elevated in cystic fibrosis CRS.

SIGNIFICANCE: Understanding the role of SPs in CRS will help develop novel treatment approaches for sinonasal pathoses.

Get full access to this article

View all access options for this article.

References

Crouch

Wright

. Surfactant proteins A and D and pulmonary host defense. Ann Rev Physiol 2001; 63: 521–554.

Sastry

Ezekowitz

. Collectins: pattern recognition molecules involved in first line host defense. Curr Opin Immunol 1993; 5: 59–66.

Crouch

Harshorn

Ofek

. Collectins and pulmonary innate immunity. Immunol Rev 2000; 173: 52–65.

Oberley

Snyder

. Recombinant human SP-A1 and SP-A2 proteins have different carbohydrate-binding characteristics. Am J Physiol Lung Cell Mol Physiol 2003; 284: L871–L881.

Woodworth

Smythe

Spicer

, et al. Presence of surfactant lamellar bodies in normal and diseased sinus mucosa. ORL J Otorhinolaryngol Relat Spec 2005; 67(4):199–202.

Woodworth

Lathers

Neal

, et al. Immunolocalization of surfactant protein A and D in sinonasal mucosa. Am J Rhinol 2006; 20(4):461–465.

Woodworth

Neal

Joseph

, et al. Surfactant protein A and D in sinus mucosa: a preliminary report. ORL J Otorhinolaryngol Relat Spec 2007; 69(1):57–60.

Benninger

Ferguson

Hadley

, et al. Adult chronic rhinosinusitis: definitions, diagnosis, epidemiology, and pathophysiology. Otolaryngol Head Neck Surg 2003; 129(suppl):S1–S32.

Bent

Kuhn

. Diagnosis of allergic fungal sinusitis. Otolaryngol Head Neck Surg 1994; 111(5):580–588.

10.

Shin

Ponikau

Sherris

, et al. Chronic rhinosinusitis: an enhanced immune response to ubiquitous airborne fungi. J Allergy Clin Immunol 2004; 114(6):1369–1375.

11.

Haczku

Atochina

Tomer

, et al. Aspergillus fumigatus-induced allergic airway inflammation alters surfactant homeostasis and lung function in BALB/c mice. Am J Respir Cell Mol Biol 2001; 25(1):45–50.

12.

Scanlon

Milovanova

Kierstein

, et al. Surfactant protein-A inhibits Aspergillus fumigatus-induced allergic T-cell responses. Respir Res 2005; 24; 6:97.

13.

Rampey

Lathers

Woodworth

, et al. Immunolocalization of dendritic cells and pattern recognition receptors in chronic rhinosinusitis. Am J Rhinol 2007; 21(1):117–21.

14.

Noah

Murphy

Alink

, et al. Bronchoalveolar lavage fluid surfactant protein A and surfactant protein D are inversely related to inflammation in early cystic fibrosis. Am J Respir Crit Care Med 2003;168: 685–691.

15.

George

White

O'Neill

, et al. Altered surfactant protein A gene expression and protein metabolism associated with repeat exposure to inhaled endotoxin. Am J Physiol Lung Cell Mol Physiol 2003; 285(6):L1337–44.

16.

Malloy

Veldhuizen

Thibodeaux

, et al. Pseudomonas aeruginosa protease IV degrades surfactant proteins and inhibits surfactant host defense and biophysical functions. Am J Physiol Lung Cell Mol Physiol 2005; 288(2):L409–418.

17.

Boggaram

Smith

Mendelson

. Regulation of expression of the gene encoding the major surfactant protein (SP-A) in human fetal lung in vitro: disparate effects of glucocorticoids on transcription and on mRNA stability. J Biol Chem 1989; 264: 11421–11427.

18.

Dulkerian

Gonzales

Ning

, et al. Regulation of surfactant protein D in human fetal lung. Am J Respir Cell Mol Biol 1996; 15(6):781–786.

19.

Wang

Yeh

Lin

, et al. Measurement of pulmonary status and surfactant protein levels during dexamethasone treatment of neonatal respiratory distress syndrome. Thorax 1996; 51(9):907–913.

20.

Lee

Kang

Woo

, et al. Upregulation of surfactant protein A in chronic rhinosinusitis. Laryngoscope 2006; 116: 328–331.

21.

Zhang

Ikegami

Dey

, et al. Reversibility of pulmonary abnormalities by conditional replacement of surfactant protein D (SP-D) in vivo. J Biol Chem 2002; 277(41):38709–38713.

22.

Kishore

Madan

Sarma

, et al. Protective roles of pulmonary surfactant proteins, SP-A and SP-D, against lung allergy and infection caused by Aspergillus fumigatus. Immunobiol 2002; 205: 610–618.

Sinonasal Surfactant Protein A1,A2,and D Gene Expression in Cystic Fibrosis: A Preliminary Report

Abstract

Get full access to this article

References