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Abstract

Objective: To investigate whether 17β-estradiol elevates prostacyclin (PGI₂) production in human myometrial cells in the middle of gestation.

Methods: The concentration of 6-keto-PGF_1α, a stable metabolite of PGI₂, in the culture medium was assessed using enzyme-linked immunosorbent assay (ELISA). Western blot analysis and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) using TaqMan (Applied Biosystems, Foster City, CA) technology were performed to evaluate the expression of cytosolic phospholipase A₂ (cPLA₂), cyclooxygenase-1 (COX-1), COX-2, and prostacyclin synthase (PGIS) in cultured human myometrial cells prepared from second trimester pregnant women (n = 3) after stimulation with 17β-estradiol.

Results: Treatment with 17β-estradiol (4-400 nM) dose-dependently elevated PGI₂ secretion from cultured human myometrial cells. Western blot analysis detected cPLA₂ and COX-1 and PGIS protein expression in the cultured human myometrial cells; however, COX-2 protein expression was below the detection sensitivity. Stimulation with 40-nM 17β-estradiol significantly up-regulated protein and mRNA expression of both COX-1 and PGIS.

Conclusion: 17βEstradiol from placenta may contribute to the augmentation of PGI₂ production in the human myometrium in the middle of gestation via up-regulation of both COX-1 and PGIS expression.

Keywords

Estrogen myometrium pregnancy prostacyclin prostacyclin synthase

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17β-Estradiol Up-Regulates Prostacyclin Production in Cultured Human Uterine Myometrial Cells Via Augmentation of Both Cyclooxygenase-1 and Prostacyclin Synthase Expression

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