Abstract
FREE TECHNICAL INFORMATION
Agilent Automation Solutions
Webinar: How to Successfully Scale PCR Workflows from Benchtop to Automation
According to Agilent, a new Webinar presents techniques for introducing reliable automation into small- and large-scale applications ranging from single cells to core laboratory applications, thereby minimizing the potential for introduction of errors into the PCR process. The webinar shares examples from the Quantitative Genomics Laboratory at the University of Texas Health Sciences Center, the Laboratory of Molecular Medicine and Global Health at Brandeis University, and Agilent Automation Solutions. It provides guidelines for when to consider moving to a liquid handling robot, what constitutes good practice for using a robot, robot use for precision pipetting for qPCR applications, scaling up from a lab bench to an automated system, setting up automated qPCR assays on 96- and 384-well plates, and the benefits of using LATE-PCR on the single-cell scale.
Application Note: Automation of qPCR Assays
According to Agilent, a new application note describes how quantitative polymerase chain reactions (qPCR) can examine a variety of housekeeping genes from cDNAs derived from different human tissues are set up on Agilent's Bravo Automated Liquid Handling Platform and by hand. Data are included demonstrating how dilutions performed by each method show similar slopes and good linearity, and targets amplified by either method give similar Ct values. On examining cDNAs from fetal and adult lungs, the authors observe differential expression in the B2M, whereas GUSB and TBP remained similar. In summary, according to Agilent Automation Solutions, the data demonstrate that the Bravo Automated Liquid Handling Platform can be useful to scientists interested in speeding up setup while maintaining reliability when performing qPCR studies.
More: www.chem.agilent.com/Library/applications/5990-4522en_lo%20CMS.pdf
Application Note: Efficient Isolation of High-Yield gDNA
This application note outlines a protocol for the Invitrogen ChargeSwitch EasyPlex gDNA kit using the Agilent gDNA isolation workstation, in which up to 10 microplates (96-well) can be processed simultaneously. The throughput of the outlined protocol is 10 min for each plate processed (depending on exact liquid handling procedures). In the described setup, microplates and tip boxes are delivered to an Agilent Bravo Automated Liquid Handling Platform by an Agilent BenchCel Microplate Handling Workstation. The Bravo gripper is used to transport microplates and tip boxes around the platform. After the washing steps, the microplates are sealed with an Agilent PlateLoc Thermal Microplate Sealer and stored in the BenchCel Workstation for downstream use. The authors show how the Agilent Bravo Automated Liquid Handling Platform has the capacity for managing the necessary reagents, plates, and tips in a compact footprint. In addition, the integration of three Agilent BenchCel stackers for tip boxes provides an uninterrupted supply of tips without user intervention. The Agilent PlateLoc Thermal Microplate Sealer securely seals the plates after processing and is easily integrated into the process workflow. Agilent VWorks Automation Control software was used to create an event-driven protocol to seamlessly integrate and reliably manage the entire process.
More: www.chem.agilent.com/Library/applications/5990-3549en_lo%20CMS.pdf
Dolomite
Study: Water-in-Oil Droplet Size Consistency
According to Dolomite, applications, such as compartmentalized chemistry and alginate polymerization, necessitate the stable formation of oil-in-water or water-in-oil droplets, and their monodispersity (size consistency) is key to obtaining accurate and repeatable results. Dolomite conducted tests to compare the size consistency of droplets formed by its Mitos P-Pump with syringe pumps. Droplets of water in oil were generated on a 100-μm-sized X-Junction, using each of the pump technologies. The droplet diameter from a continuous stream was measured using image analysis software. The resulting data demonstrate that the Mitos P-Pump provides a coefficient of variation in the droplet diameter of less than 0.6%, compared with 5.5% for the leading syringe pump.
Fluidigm Europe
Application Poster: Genotyping SNPs
A new poster from Fluidigm Europe describes how its BioMark System and 96.96 Dynamic Array integrated fluidic circuit (IFC) are used by the University of Washington for verification and genotyping of SNPs in Alaskan populations of chum salmon. It describes the development of new melt curve analysis methods on four closely related chum salmon populations to verify putative SNPs through next-generation sequencing. Using the described methodology, the researchers produce a set of verified SNPs that can be tested across all chum salmon populations. Ninety-six samples are run simultaneously against 96 SNP genotyping assays for a total of 9216 genotypes on a single chip. The conclusion is that the Fluidigm platform is well suited for high-throughput SNP genotyping.
More: www.fluidigm.com/pdf/posters/SNPIII%20-%20Chum%20Poster%20Mar10.pdf
INTEGRA Biosciences and Insphero
Application Note: Fast and Economic Three-Dimensional Microtissue Cultivation
A new application note from INTEGRA and Insphero describes how Insphero's unique hanging-drop cell culture system is used to generate a diverse range of microtissues. This microtissue system is based on the GravityPlus culture platform, for which multichannel pipettes have to be used for the efficient liquid media exchange of the culture drops. The researchers demonstrate how INTEGRA Viaflo electronic pipettes allow fast and economic cultivation of the cells in hanging droplets. According to INTEGRA, this application is an example of the unmatched multichannel dispensing precision achievable using INTEGRA Viaflo pipettes with the GripTip system.
More:
Micronic Europe
New Web Site
In addition to production and other company information, Micronic's new Web site includes product video demonstrations, a database of application notes, the Micronic Knowledge Centre, and the 2010—2011 educational webinar program.
More: www.micronic.com
APPLICATION AREAS
A2 Technologies
Rock and Mineral FTIR Diffuse Reflectance Spectral Libraries
Two new diffuse reflectance mid-infrared spectral libraries of rocks and minerals extend the utility of A2's Exoscan and FlexScan handheld FTIR analyzers for geoscience applications. The first library contains spectra of general rocks and minerals sourced from around the world, whereas the other is focused on commercially important or strategic minerals.
More: www.a2technologies.com
American Type Culture Collection
Primary Adipose-Derived Mesenchymal Stem Cell Growth Solutions
American Type Culture Collection (ATCC) now offers a complete solution to successfully grow normal human primary adipose-derived mesenchymal stem cells, which includes stem cell differentiation, tissue engineering, cell therapy, regenerative medicine, and the creation of induced pluripotent stem cells (PSCs). Each lot of ATCC normal human primary adipose-derived mesenchymal stem cells is tested for sample purity and cryopreserved in the second passage to ensure high viability and plating efficiency.
More: www.atcc.org
AMS Biotechnology
Expression-Ready Lentiviral Particles
According to AMS Biotechnology (AMSBIO), this new range of ready-to-use lentiviral particles delivers genes in a highly reproducible and controllable manner for the expression of more than 250 human proteins, mouse proteins, enzymes, and fluorescent markers. Using protocols based on co-expressed red fluorescent protein by 2A peptide processing (SureTiter) or RSV promoter (inducible system), AMSBIO lentiviral particles can enable real-time monitoring of protein expression. Suggested applications include gene delivery for expression or knockdown in hard-to-transfect cell types, such as primary cells, stem cells, drug-arrested cells, and cells in suspension.
They also can create stable cell lines for long-term high constitutive expression or Tet-inducible expression applications in the presence of TetR (a repressor protein), and they can be used in vivo for generating transgenic animals and inducing PSC (iPSC). Custom lentiviral particles also can be generated.
More: www.amsbio.com/expression-ready-lentiviral-particles.aspx
CometAssay Neutral Control Cells
To consistently optimize neutral Comet assays for highly reproducible results and standardize neutral electrophoresis methods between individual users and laboratories, AMSBIO has added CometAssay Neutral Control Cells for its CometAssay Electrophoresis System to its already established standardized procedure and reagents for the alkaline CometAssay.
Solu BL21 Competent Escherichia Coli
This new BL21 host strain for soluble mammalian protein expression uses a directed evolution approach to develop a mutant strain of BL21(DE3) E. coli that can produce soluble protein in most of the cases where expression in the parent BL21(DE3) yields no detectable soluble product. The Solu BL21 is compatible with all T7 promoter-based expression vectors.
More: www.amsbio.com/express-insoluble-proteins-in-soluble-form-SoluBL21.aspx
New Kits for Proteoglycan Research
Glycosaminoglycans (GAGs) are polysaccharides consisting of repeated dimers. All but hyaluronic acid (HA) possess sulfate bases and exist in living organisms in the form of proteoglycans. Sulfated GAGs play important roles in the functions of a number of tissues.
A new sulfated GAG quantitation kit allows easy quantification of sulfated GAGs in tissues, such as cartilage, cultured chondrocytes, and chondrocyte culture solution, and is considered ideal for use in cartilage research and other fields. Based on a colorimetric determination method, using 1,9-dimethylmethylene blue, the new kit is capable of measuring amounts in the 2.5- to 80-μg/mL range within a wide standard curve. Provided in a standard 96-well-plate format that can be stored for up to 1 year, the kit facilitates simple, simultaneous processing of multiple samples.
A new assay kit for screening of hyaluronidase inhibitors and quantification of hyaluronidase activity in cells and other biological fluids also is available.
Hyaluronidases are a group of enzymes that degrade HA. HA is widely distributed in the body and forms one of the most versatile macromolecules of the extracellular matrix. Traditionally, measurement of the activity of hyaluronidase enzymes has been difficult to perform and standardize. The new assay kit from AMSBIO is non-radioactive, fast, and easy to perform; sensitive and specific to hyaluronidase; and uses a universal 96-well-plate format.
AMSBIO also has added a selection of new standards to its range of products for glycobiology and glycoanalysis. The new standards include oversulfated chondroitin sulfate, dermatan sulfate, HA from porcine skin, and heparan sulfate from porcine mucosa.
BAC
CaptureSelect HumanPlasma14
For highly specific, reproducible depletion of the 14 most abundant proteins from human blood plasma or serum, HumanPlasma14 is designed for single-step, single-use depletion of more than 99% of the abundant proteins. The resulting sample can then be used for further downstream applications, including gel electrophoresis and mass spectrometry. HumanPlasma14 is the first product from BAC's CaptureSelect Proteomics Toolbox that has been proven in laboratory and large-scale industrial applications and can be custom combined for specific protein depletion needs. The slurry can be prepacked into columns or used in custom-made formats.
More: www.captureselect.com/research-products/proteomics-sample-preparation/
BioFocus
PPI01 and FFG01 Compound Libraries
According to BioFocus, its new protein—protein interaction library (PPI01) is designed around a scaffold that mimics a “beta-sheet type” hydrogen bonding pattern, a motif observed in protein—protein interactions. The new FieldFocus GPCR Library (FFG01) is designed using field-and shape-based overlays of known ligands to generate a library that targets the allosteric site of metabotropic glutamate receptors, a subtype of Family C GPCRs.
More: www.biofocus.com
Biotage
IST ISOLUTE QuEChERS
According to Biotage, these new dispersive SPE (dSPE) products offer Quick, Easy, Cheap, Effective, Rugged, and Safe extraction of pesticides for multiclass, multiresidue pesticide analysis in vegetables and fruits. The 15-mL extraction and cleanup tubes contain sufficient sorbent to process 10- or 15-g samples according to the AOAC and EN methodologies and allow for immediate sample mixing and transfer to larger 50-mL sample tubes. The shipping package contains 25 of the 15- or 50-mL tubes and serves as a disposable rack, eliminating the need to open individually sealed packages.
More: www.biotage.com/quechers
Cell Signaling Technology
PathScan Signaling Nodes Multiplex IF Kit #8999
This new multiplex assay allows users to simultaneously assess signaling through key pathway nodes using automated imaging or laser scanning high-content platforms or manual immunofluorescence microscopy. This kit allows the analysis of multiple pathway endpoints within a single sample and contains a cocktail of three primary antibodies, optimally formulated for use in conjunction with the provided Alexa Fluor detection cocktail. The kit provides reagents necessary to perform 100 assays (based on 100-μL volume).
More: www.cellsignal.com
PhosphoPlus In-Cell Duets
These new ICW-compatible PhosphoPlus In-Cell Duets provide a way to assess protein activation status in whole-cell assays using the LI-COR Biosciences Odyssey Infrared Imaging System. These kits contain an activation state and total protein antibody to a target of interest, allowing levels of phosphorylated protein to be independently measured and normalized to total protein concentration. The selected antibodies are based on superior performance and are validated to work in a multiplex assay format. A near-infrared (IR) detection cocktail is also included.
More: www.cellsignal.com
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb (PE Conjugate) #5682
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb #4370. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb (PE conjugate) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and when singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
Phospho-NDRG1 (Thr346) (D98G11) XP Rabbit mAb #5482
Phospho-NDRG1 (Thr346) (D98G11) XP Rabbit mAb detects endogenous levels of NDRG1 when phosphorylated at Thr346. This monoclonal antibody likely cross-reacts with other conserved phosphorylation sites on NDRG1 at positions Thr356 and Thr366. The antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr346 of NDRG1.
Di-Methyl-Histone H3 (Lys79) (D15E8) XP Rabbit mAb #5427
Di-Methyl-Histone H3 (Lys79) (D15E8) XP Rabbit mAb detects endogenous levels of histone H3 only when dimethylated on Lys79. This monoclonal antibody does not cross-react with nonmethylated, monomethylated, or trimethylated Lys79. In addition, the antibody does not cross-react with methylated histone H3 Lys4, Lys9, Lys27, Lys36, or methylated histone H4 Lys20. The antibody is produced by immunizing animals with a synthetic peptide containing histone H3 di-methyl lysine 79.
Iκα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor 488 Conjugate) #5743
This Cell Signaling Technology antibody is conjugated to Alexa Fluor 488 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814. IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor 488 Conjugate) detects endogenous levels of total IκBα protein. This monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding to the amino terminus of human IκBα.
Livin (D61D1) XP Rabbit mAb #5471
Livin (D61D1) XP Rabbit mAb detects endogenous levels of total livin protein. Livin is a potent anti-apoptotic IAP family member containing a single BIR domain and RING finger motif that is highly expressed in human melanomas and absent in normal tissues. Livin (D61D1) XP Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala195 of human livin protein.
Platelet-Derived Growth Factor Receptor α (D13C6) XP Rabbit mAb #5241
Platelet-derived growth factor (PDGF) receptor α (D13C6) XP Rabbit mAb detects endogenous levels of PDGF receptor α protein. This monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the PDGF receptor α extracellular domain.
Ezh2 (D2C9) XP Rabbit mAb #5246
Ezh2 (D2C9) XP Rabbit mAb detects endogenous levels of total Ezh2 protein. Enhancer of zeste homolog 2 (Ezh2) is a member of the polycomb group of proteins that are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis. Ezh2 (D2C9) XP Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein.
Fluidigm
FR48.48 Dynamic Array Integrated Fluidic Circuit
According to Fluidigm, its new FR48.48 Dynamic Array IFC is the first reusable bio-chip for the SNP genotyping market for a wide range of applications, including plant and animal biotechnology. The reusable chip can decrease the cost per genotype by up to 80% for ultra-high-throughput SNP genotyping compared with Fluidigm's single-use 48.48 Dynamic Array IFC, and it can support an accelerated workflow for higher sample throughput applications. Each chip can be used up to five times and can produce results for 48 samples across 48 assays for a total of up to 11,520 genotypes over the five uses. Fast loading and thermal cycling allow a single operator to run more than 3 × the number of samples in a day compared with that using single-use 48.48 Dynamic Array IFCs. The reusable chip provides 99% or greater call rates and 99.75% or higher accuracy. The launch of the reusable FR48.48 Dynamic Array IFC is accompanied by the introduction of the IFC Controller WX, a device that automatically performs fast loading or cleaning of this reusable IFC. The reusable chip can be loaded in 30 min, and the overall time for loading and thermal cycling is about 1 h, compared with approximately 3 h for single-use 48.48 Dynamic Array IFCs.
More: www.fluidigm.com
Oxford Gene Technology
CytoSure ISCA Uniparental Disomy 4 × 180k Array
This new array offers the ability to simultaneously detect DNA copy number variation using Oxford Gene Technology's (OGT) ISCA consortium—endorsed 4 × 180k aCGH array, along with whole-chromosome uniparental disomy (UPD) using SNP probes, on a single array. OGT's UPD detection capability is enabled by multiple rounds of SNP probe selection and validation, targeting over 6000 SNPs with evenly distributed probes, and the combined ISCA—UPD array ensures nearly identical labeling and hybridization conditions to standard aCGH. In addition, the aCGH protocol is largely unaltered and any reference DNA can be used.
More: www.ogt.co.uk
QIAGEN
Cancer Companion Diagnostics
The new CE-marked tests from QIAGEN with the Rotor-Gene Q thermocycler (EGFR) and the PyroMark pyrosequencing system (EGFR, NRAS, KRAS, BRAF) are for use in personalized health care of cancer by identifying certain mutations in these particular genes. The kits are offered as CE-IVD-marked therascreen tests in Europe and globally for research use only.
The PCR-based kits can be used in combination with the QIAsymphony RGQ instrument that allows automation of the complete workflow from sample preparation and assay setup to detection with the Rotor-Gene Q thermocycler. Pyrosequencing kits can be automated with the QIAsymphony series.
More: www.qiagen.com
Roche Diagnostics
STAT Immunoassays for Cardiac Biomarker Testing
With a 9-min duration, STAT immunoassays for cardiac biomarker testing on the cobas 6000 analyzer series are considered faster than any other cardiac immunoassay tests currently available on an integrated platform. The tests complement a line of existing STAT cardiac assays for Roche's standalone platform, the cobas 4000 series. The new tests include troponin T, troponin I, CK-MB, myoglobin, and NT-proBNP, and run on the cobas e 601 analyzer, part of the cobas 6000 analyzer series. The cardiac STAT assays can all be run with one simple blood draw by means of the Roche OneTUBE solution, which consists of a single lithium heparin tube that can be run on any of Roche's integrated platforms.
More: www.roche-diagnostics.us
SeraCare Life Sciences
Seroconversion Panels
Each of the six new human immunodeficiency virus (HIV) seroconversion panels consists of a set of undiluted plasma samples from a single plasma donor during a period of HIV seroconversion and is ready to be used in vials of 1.0 mL per patient sample. Data sheets for each panel list results from 20 U.S. and international test methods for markers of HIV infection, including HIV RNA, first- to fourth-generation screening tests for HIV, confirmatory tests, and a rapid discriminatory method. A seroconversion graph of critical data points is also included. Each panel consists of undiluted plasma samples from serial collections from a single deferred plasma donor during very early HIV infection.
More: www.seracarepanels.com
Thermo Fisher Scientific
Nunclon Vita Stem Cell Culture Surface
According to Thermo Fisher Scientific, this new, energy-treated polystyrene surface enables direct attachment, colony formation, and growth of human embryonic stem cells (ESC) and induced PSCs (IPS), without the need for feeder cells or matrix coatings. In media supplemented with a Rho-associated kinase (ROCK) inhibitor, human ESCs can be cultured on the Nunclon Vita Surface for at least 10 passages without any loss of pluripotency or karyotype changes. By eliminating matrix coatings and feeder cells, the Nunclon Vita surface reduces culture variability and is less labor-intensive. The Nunclon Vita surface treatment is considered more adaptable for scalable cell expansion as cells grow directly on the polystyrene surface. In addition, the new surface works in synergy with a ROCK inhibitor for expansion of human ESC, IPS, and human embryonic kidney cells to enable enzymatic and mechanical passage protocols.
AUTOMATED SYSTEMS
Biotage
Isolera UV-VIS Flash Purification System
This new system enables detection of organic molecules with spectral absorbance between 200 and 800 nm. The system provides detection capability to near-IR range. Applications for the UV-VIS system include natural products (carotenoids, flavanoids, proanthocyanidins, and others); synthetic dyes; diagnostic dyes; and OLED chemicals. The new system reduces solvent use up to 60% by converting linear gradients to optimized step gradients; the quatro-binary gradient capability enables use of up to four solvents in a single binary gradient; and TLC-to-gradient capability simplifies method transfer from TLC. The system is also optimized for use with universal fit, SNAP flash purification cartridges, and default methods are designed around both TLC conditions and cartridge sizes.
More: www.biotage.com
GE Healthcare
ÄKTA Avant 150 LC System
With flow rates up to 150 mL/min, the ÄUKTA avant 150 liquid chromatography system is designed for process development and scale-up to ensure smooth transition from discovery to full-scale production. The UNICORN 6.1 control software enables seamless method transfer between ÄUKTA avant 25 and ÄUKTA avant 150. UNICORN 6.1 also comes with integrated Design of Experiments functionality. ÄUKTA avant includes several features for optimal operational security, such as a cooled fraction collector and traceable column handling. ÄUKTA avant also supports intelligent packing of AxiChrom columns for predictable scale-up to production scale.
More: www.gelifesciences.com/aktaavant or www.youtube.com/watch?v=JtNZllv_2Qs
Manz Automation
sciLine.speed Laboratory Robotics Platform
Manz's sciLine.speed is now available with modules for sample preparation and purification: weighing, dissolving, filtration, and chromatography—all in less than 0.8 ×1.2 m of laboratory space. The new combination of ultrasound and shaking and the parallel filtration module now enable simultaneous sample preparation jobs. In combination with the optional Microdos powder dispensing module, the fully automated preparation of analytic vials, screening plates, and other solid or fluid sample formats also is possible.
More: www.manz.com
sciLine.store Substance Storage
The sciLine.store storage solution now can store substances in an area smaller than 2 ×3 m using miniaturized robotics for storing laboratory samples and removing them from storage. The sciLine.store can identify and weigh different vial formats, offers manual access to storage racks, and can be connected to a user's IT infrastructure. An optional cooling system and additional storage modules also are available.
From: www.manz.com
Qiagen
QIAsymphony RGQ
This new and complete workflow solution comprises modules that can be used as standalone systems or combined into a fully integrated system. It includes the physical and data integration of the modules QIAsymphony SP for sample preparation, QIAsymphony AS for assay setup, and the Rotor-Gene Q real-time PCR thermocycler. QIAsymphony also provides a broad PCR-based assay menu, including the artus real-time PCR kits for the detection of HIV, HCV, HBV, and a transplantation panel with CMV, EBV, HSV, VZV, and BKV. A large pipeline of further tests will be launched soon, including therascreen EGFR, KRAS, as well as artus Influenza, CT, CT/Ng, and an extended transplantation panel.
The complete CE-IVD-compliant workflow of the standardized system enables reliable pathogen detection with the flexibility to process multiple input formats and a varying number of 1–72 samples per run. The QIAsymphony RGQ also offers workflow optimization through the option for parallel runs and flexible batch sizes and intuitive, easy-to-use software.
The Automation Partnership
ambr Micro Bioreactor System
This new version of the ambr workstation automates the operation of 48 disposable micro-bioreactors and closely replicates the characteristics of benchtop bioreactors at microscale (10- to 15-mL working volume). The 48 micro-bioreactors are arranged in four culture stations containing sets of 12, each with a miniature internal impeller, as well as individual closed-loop control of dissolved O2 and pH and a supply of gases. Each culture station has independent stirring speed and temperature control, allowing scientists to test up to four different temperatures and stir speeds simultaneously. The new ambr system controls culture setup and inoculation; the addition of alkalis and feeds; as well as culture sampling for cell count and analysis, such as antibody titre, and is less than 50 cm wider than the original ambr to fit in a standard laboratory biosafety cabinet.
TTP LabTech
High-Throughput Neutrophil Chemotaxis Studies
A new system comprising the Acumen eX3 microplate cytometer from TPP LabTech and the iuvo Chemotaxis Assay Plate from BellBrook Labs can achieve approximately fourfold time savings compared with a similar method using automated microscopy and offline data processing. The Acumen eX3 is a laser scanning microplate cytometer for the analysis of fluorescence in cell-based assays. In addition to offering enhanced multiplexing functionality, Acumen eX3 has a wide field of view that enables whole-well and microchannel scanning. The iuvo Chemotaxis Assay Plate is a high-content, automation-friendly microconduit array plate that facilitates horizontal chemotaxis in the presence of a defined gradient. The combination of Acumen eX3 with the iuvo microchannels results in a rapid and robust approach to neutrophil chemotaxis studies.
Uniqsis
FlowSyn Auto-LF
The new FlowSyn Auto-LF automated loop filling module is designed to enhance the FlowSyn system by allowing flow chemists to perform multiple experiments with multiple reagents automatically with simultaneous loop filling and fraction collection. It can prepare focused combinatorial libraries in flow and perform automated experiment optimization and reagent screening. Because the FlowSyn Auto-LF uses a separate liquid handler and fraction collector, sample loop filling for a subsequent experiment can take place before a current experiment is finished. There are no injection ports to leak or block, and fully integrated wash protocols minimize the risk of cross-contamination. Partial filling of loops enables stoichiometry to be varied in subsequent experiments, and volumetric accuracy is ensured by sampler calibration to position samples precisely in the loops. Data monitoring is included, and reaction progress can be monitored in real time and saved as a log file.
More: www.uniqsis.com/products/flowsyn-continuous-flow-reactor-1
INSTRUMENTATION AND HARDWARE
Bio-Rad Laboratories
Gel Doc EZ
According to Bio-Rad, this new compact gel documentation system provides publication-quality images and analysis in seconds with the push of a button for researchers who perform DNA, RNA, and protein electrophoresis, and western blotting. The expandable Gel Doc EZ imager eliminates the need for manual manipulation of filters, lenses, or lighting. The imager allows the use of multiple application-specific trays, including a ultraviolet (UV) tray for imaging fluorescent stains, such as ethidium bromide; a white tray for imaging colorimetric stains, such as coomassie blue; a blue tray for imaging SYBR green—stained DNA; and a stain-free tray for imaging proteins separated by electrophoresis on Bio-Rad's Criterion TGX Stain-Free precast gels.
More: www.bio-rad.com
Thermo Fisher Scientific
Piko and Arktik Thermal Cyclers
According to Thermo, its compact Piko Thermal Cycler provides thermal performance, fast ramping rates, and quick settling times for PCR efficiency and well-to-well consistency. The Piko utilizes ultra-thin-walled vessels for better temperature transfer, which enables protocols to be completed more quickly. Available in 24- and 96-well formats, the Piko Thermal Cycler can use standard low-profile PCR tubes, eight tube strips, as well as specialized 24- and 96-well Piko PCR plates. The Arktik Thermal Cycler provides three interchangeable blocks for standard 96-, 384-, and 2 ×48-well PCR plates, with a broad and accurate temperature gradient option (not available in Germany or the United States) offering a cross-block differential of up to 30 °C. Both the Piko and Arktik Thermal Cyclers feature intuitive user interfaces, with clear display of protocols.
More: www.thermoscientific.com/piko or www.thermoscientific.com/arktik or www.thermoscientific.com/pcrsolutions
BioTek Instruments
Synergy H1 Hybrid Multi-Mode Microplate Reader
The Synergy H1 is a monochromator-based multimode microplate reader for UV-Vis absorbance, top and bottom fluorescence, and luminescence assays that can be upgraded to a hybrid reader with the addition of a high-performance filter module. This independent filter module with dichroic mirrors supports fluorescence polarization, time-resolved fluorescence, TR-FRET and BRET assays with ultra-high sensitivity. Synergy H1 includes BioTek's Gen5 Data Analysis Software and is compatible with BioTek's Take3 Multi-Volume Plate that measures up to sixteen 2-μL samples, two BioCells, or a standard cuvette for multivolume, multisample analysis.
More: www.biotek.com
MultiFlo Microplate Dispenser
According to BioTek, its new all-in-one MultiFlo Microplate Dispenser uses multiple technologies to dispense multiple reagents into multiple microplate types. It dispenses up to four independent, parallel reagents in volumes from 1 to 3 mL and accommodates microplates from 6 to 1536 wells, including low-profile, standard-height, deep well and even microtubes. Two integrated peristaltic pumps provide low prime volumes, full reagent recovery, and high chemical compatibility, whereas two integrated syringe pumps feature program-and-forget solutions without need for instrument recalibration or consumables. Fully modular architecture means that any dispensing combination may be chosen and upgraded as necessary. Dispense flow rates are fully adjustable from high-velocity media exchanges to gentle washing for delicate cell-based applications. Dispense protocols may be created and run through the keypad interface or through BioTek's LHC Software. Combined with BioTek's BioStack Microplate Stacker, MultiFlo may be programmed for unattended dispensing of up to 75 microplates.
More: www.biotek.com
Dolomite
Mitos Sensor Units
Mitos Sensor Units are for measuring and displaying pressure and flow rates in microfluidic systems. With a real-time display and low internal volumes, each sensor is designed to minimize interference with the liquid flow. All data are displayed on the Mitos Sensor Display Unit, which interfaces with each of the sensor units using a push-and-click action. Comprising of five flow rate sensors and one pressure sensor (-0.5–30 bar), the complete range is interchangeable, and each device can run standalone or integrated with a Mitos P-Pump for the efficient logging of flow rate output. All data are easily transferred to a PC through USB.
ForteBio
Dip and Read Protein G Biosensor
For rapid detection and quantification of numerous types of mammalian immunoglobulin (IgG), this new biosensor runs on the ForteBio's label-free Octet instrumentation platform and enables researchers to obtain highly specific binding information in real time. It can be regenerated and reused, and it comes with recombinant protein G pre-immobilized on the surface.
More: www.fortebio.com
Harvard Apparatus
PHD ULTRA Nanomite Syringe Pump
This new light-weight pump features foot pedal activation for hands-free injections. User-defined methods can be accessed by means of an LCD touch screen. Although designed for fast bolus infusions with high accuracy and precision, the PHD ULTRA Nanomite can deliver low nanoliter injections up to tens of mL/min, particularly when combined with Harvard Apparatus microneedles as small as 37 gauge. The PHD ULTRA has flow ramping capability for spatially continuous volume injections into organs, muscle tissues, cells, and neurons, with minimal physiological impact.
More: www.harvardapparatus.com
Petrotest Instruments
Petrotest PA5 Digital Manometer
In combination with PetroNet software, the Petrotest PA5 Digital Manometer offers a data logging and management solution for determining the oxidation stability of fuels, lubricants, and oils. It can simultaneously record temperatures along with pressure and time data. For continuous real-time monitoring of bath temperatures, the Pt1000 with a resolution of 0.1 °C and a range of up to 200 °C is recommended. For ASTM D525/ISO 7536, PetroNet software provides a temperature correction algorithm. For fuel oxidation and other ASTM oxidation tests, the PA5 offers predefined routines and settings to ensure that tests are conducted according to international standards. When using the professional version, the test stop can be programmed based on criteria, such as elapsed time, pressure drop, or percentage of pressure drop.
Although the PA5 manometer performs the actual data sampling, the PetroNet application software manages online configuration, data transfer, and storage to a computer or network. Test parameters can be modified on-screen. When used in an autarkic mode (such as in a hazardous environment) pressure, temperature, and time readings are retrieved from the PA5 after the test and imported into PetroNet through USB or RS232 communications. In addition, the PA5 manometer features selectable pressure (bar, psi, or kPa) and temperature (°Cor °F) units and allows for recording up to 57,000 paired readings at intervals shorter than 15 s. Pressure ranges from 0 to 14 bar rel. are covered.
More: www.petrolab.com
Porvair Sciences
Ultravap Microplate Evaporator
According to Porvair, its Ultravap is now refined to facilitate integration with laboratory robots, to increase operational versatility, and to enable simple safe venting of removed solvents. The plan form of the leading edges of the unit are redesigned to allow robot gripper arms an extra 22 mm of linear access to the evaporation deck. This allows the gripper to handle heavy deep well blocks with ease and position them under the evaporation head more precisely. In addition, Porvair has made available a set of side panels that fit on either side of the unit to ensure that exhaust gas—carrying solvent fumes can be easily extracted either to the front or rear of the unit. To facilitate easy connection of the rear vent to a standard fume extraction duct, a new optional adaptor allows direct connection of a 4-in.-diameter fume duct to the evaporator.
More: www.porvair-sciences.com
Syrris
Atlas Potassium Pressure System
According to Syrris, this modular 3-bar pressure reactor can be controlled automatically or manually and supplied with glass vessels ranging from 100 mL to 3 L that can be changed in less than 1 min. With overhead stirring and a temperature range of −80 to +200 °C, the basic system provides automatic temperature and stirrer control and pressure sensing. PED/ASME compliance, a burst disc, and pressure relief valve contribute to safety. Modes of operation include manual pressure control, automatic pressure maintenance, pressure control with mass flow monitoring, mass flow control, and off-gas monitoring. Control by means of the Atlas base enables simple logging of data to a .csv file for download to a USB stick. Optional PC control offers advanced functionality, including the complete automation of all parameters, alarms, and real-time graphs.
More: www.syrris.com
TandD Corporation
NEW RTR-574 Wireless 4 Channel Logger
This new logger monitors illuminance, UV, temperature, and humidity, and is also capable of displaying cumulative illuminance and cumulative amount of UV light in the LCD display. Its wide luminosity range covers 0–130k lx and low light resolution to 0.01 lx. The UV range covers 0–30 mw/cm2. Internal accumulation of exposure is for both light and UV. The RTR-574 has a range from 10–99% relative humidity along with a temperature range from 0 to +55 °C. It operates on one AA battery and can store up to 8000 readings times four channels for a total of 32,000 readings in one-time or endless recording mode.
More: www.tandd.com
The Automation Partnership
Fill-It 48-Way
The Fill-it 48-way is the newest system in TAP's range of benchtop automated dispensers and is compatible with Nunc's new racks of 2.0- and 5.0-mL vials, supplied in racks preloaded with 48 vials. With these bigger vial volumes, Fill-it 48-way allows processing of larger batches. It can be integrated with many liquid handlers and can fit in a standard laboratory tissue culture cabinet to maintain aseptic conditions.
Biological Safety Cabinet for Fill-It
A new mobile safety cabinet configured specifically for the Fill-it automated tube filling system features airflow and biological safety performance standards of a class II biosafety unit and is designed for user convenience. It comes with casters and variable height adjustment for moving between laboratories. The cabinet maintains continuous airflow when Fill-it is in situ, and its specially configured sliding glass door also allows fully protected access to the system. Cabinet materials are compatible with decontamination using hydrogen peroxide.
LABWARE AND LABORATORY SUPPLIES
Air Science
Purair ECO Ductless Fume Hoods
Available in five sizes from 30 to 69-in. wide, the Purair ECO line of energy-efficient ductless fume hoods is designed for chemical and particulate protection over a broad range of applications. The Purair ECO is available with a choice of controllers and an optional BACnet network interface to connect all cabinet control, monitoring, and alarm functions to an open-source facility monitoring system.
More: www.airscience.com
Beckman Coulter
Krome Orange
According to Beckman Coulter, this violet-excited organic dye expands the range of available choices and dye sensitivity limits for violet lasers. Common gating markers can be transferred to this fluorochrome, freeing other channels for use with more esoteric markers and enabling more versatile 10-color flow cytometry applications. Krome Orange has excitation and emission maxima of 398 and 528 nm, respectively, and provides a brighter signal than Pacific Orange conjugates with equivalent spectral overlaps into adjacent channels. The new dye is at least as bright as V500 and can provide more than twice the population separation obtained with Pacific Orange dye conjugates, with little compensation compared with Pacific Blue dye. Krome Orange conjugates show optimal performance with violet laser excitation and a 550/40 bandpass filter (the standard FL10 channel on flow cytometry systems), whereas no excitation is detected using a 488-nm laser. The initial release of Krome Orange includes conjugates to human CD45 and CD4.
More: www.beckmancoulter.com
Biotage
Resolux HPLC Columns
According to Biotage, this new line of HPLC columns delivers purification solutions for very short peptides up to large chains by offering consumables in three different pore sizes. The line is available in three different surface chemistries, covering the range from hydrophobic to hydrophilic peptides. They include analytical, semi-preparative, and preparative HPLC columns from 2.1- to 30-mm diameter, for predictable scale-up from microgram to multigram scale.
More: www.biotage.com/peptides
BMG Labtech
SPECTROstar Nano
According to BMG LABTECH, its new SPECTROstar Nano is an ultrafast, full spectrum absorbance microplate reader with a cuvette port for all absorbance assays. Its rapid full-spectrum analysis at a resolution of 1 nm allows for absorbance assays that were never before possible on a microplate reader.
It can capture a full UV-Vis spectrum from 220 to 1000 nm in less than 1 s/well and measure sample volumes down to 2 μL. The built-in cuvette port can be used for kinetic studies and quick experiments and measure all standard microplate formats up to 1536 wells. The SPECTROstar Nano allows for single push-button operation for basic commands and specific assay protocol setup. Other features include multimode shaking, incubation, kinetic readings, well scanning, automated path length correction, and a gas vent.
More: www.bmglabtech.com
GE Healthcare
HiScale Pressure-Stable Columns
Optimized for process development and preparative chromatography, these empty columns are available in inner diameters of 16, 26, and 50 mm and in lengths of 20 and 40 cm. The columns are pressure stabile up to 20 bar, ensuring compatibility with modern BioProcess media, such as MabSelect and Capto. The ability to axially compress the gel bed and a nonrotating plunger mechanism enable a wide range of packing protocols. The measurement scale on the column and its design enable precise packing. The QuickLock mechanism of the end caps facilitates column handling and cleaning. HiScale can be used with nearly all organic solvents commonly used in liquid chromatography of macromolecules.
More: www.gehealthcare.com
Kimble Chase
KimCote Plastic Coated Glassware
According to Kimble Chase, its new KimCote is an ultra-clear coating that is applied to the exterior of laboratory glassware to resist breakage, to contain leakage, and to provide a nonslip surface texture for an improved grip when wet or dry. KimCote coating is available on many Kimble Chase products, and application of KimCote coating to other glassware is available on request. All KimCote glassware are designed to withstand steam autoclave temperatures up to 120 °C (250 °F) and freezing temperatures up to −20 °C(−4 °F). KimCote is safe for dishwashers and microwaves, and the coated surface accommodates labeling and marking.
More: www.kimble-chase.com
Thermo Fisher Scientific
NESLAB ThermoFlex Series of Recirculating Chillers
The ThermoFlex 24000 is the latest addition to this product platform and has a cooling capacity of 24,000 watts for higher levels of cooling performance. Each chiller includes water filters that can be changed while the unit is in operation and an intuitive user interface for ease of operation.
Syncronis HPLC Column Range
According to Thermo Fisher Scientific, this new Syncronis column range is engineered to deliver exceptional reproducibility by providing highly pure, high-surface-area silica; dense bonding; and double endcapping, all controlled and characterized through rigorous testing. New enhanced, automated column packing methods used within the Syncronis range deliver greater consistency, and every column is individually tested to ensure that it meets the required specifications. Syncronis columns are available in 5- μm particle size for conventional HPLC applications and in 1.7-μm particle size for high-speed, high-efficiency UHPLC separations.
La-Pha-Pack Crimping Tools
For a controlled, low-effort method of vial sealing, these new stainless steel cleanroom crimping tools are available in sizes ranging from 11 to 20 mm to provide optimal crimp for a variety of vial sizes. Pressure and height are adjustable to accommodate vial styles. These crimping tools can withstand dry heat sterilization or autoclaving and are considered ideal for sensitive chromatography cleanroom applications.
More: www.la-pha-pack.com
Torrey Pines Scientific
EchoTherm IC20XR Chilling/Heating Dry Bath
The Peltier-driven EchoTherm IC20XR can freeze, chill, or heat samples from −10 to 110 °C in assay plates, centrifuge tubes of all sizes, vials, and most test tube sizes. It has digital display and control of temperature to 1 °C; countdown timer in days, hours, minutes, and seconds, to 30 days; data logger; and RS232 interface to control the unit by computer or to record data.
MICROPLATES
Porvair Sciences
MicroBurst Clear Sealing Film
With its adhesive encapsulated in millions of tiny spheroids, MicroBurst sealing film, when first handled, is not sticky and can be quickly and easily moved into position. Pressing down hard when the film is in the correct place bursts and activates the adhesive microcapsules. According to Porvair, the film has excellent optical properties and produces a very firm seal that quickly strengthens to form a near-permanent seal on your plates. MicroBurst film can be pierced by pipette tips or robot liquid handlers and is temperature stable.
More: www.porvair-sciences.com
INFORMATICS AND COMPUTING
FEI Company
Software Solutions
The new software upgrades are part of FEI Company's automated mineralogy technology suite, a high-throughput analysis solution that provides quantitative mineralogical data on a wide range of geological samples. The Mineral Liberation Analyzer (MLA) v3.0 measurement and analysis suite has been completely revised. The user interface is enhanced with flexibility in processing of particle images and extraction of quantitative mineralogical data. A completely new and integrated data management system and simplified measurement setup and mineral standards management protocols also have been introduced. The iDiscover v5.0 is the latest upgrade to the QEMSCAN software suite and includes an automated mineral identification algorithm. Users can now directly identify more than twice as many elements, which enables more subtle discrimination of complex mineral compositions. The new QEMSCAN, now available on the FEI Quanta 650 SEM platform, has enhanced image quality and faster X-ray acquisition throughput.
Manz Automation
Manz Workflow Manager
The new Manz Workflow Manager manages local laboratory processes and closes the gap between laboratory equipment, chromatography data systems, and LIMS. It is the laboratory equivalent of Manufacturing Execution Systems. It communicates with LIMS and ERP systems. Users can define specific processes and link them to sample data. The Workflow Manager controls the generated tasks across all connected devices. Samples are identified by each connected device by barcodes and are automatically processed using the appropriate method. The resulting data are saved in the Workflow Manager's database and can be exported as a printed report or as an export file for a LIMS or ERP system. Typical areas of application include sample preparation and analysis, chromatographic purification, and compound logistics.
More: www.manz.com
Two Fold Software
Qualoupe LIMS Specialist Software Applications
According to Two Fold Software, its Qualoupe LIMS solution has been enhanced to enable contract laboratories to generate invoices that comply with UKAS accreditation requirements, accept customer samples remotely online, and allow customers to access results remotely online. New, flexible reporting capabilities enable customer-specific reports.
More: www.twofoldsoftware.com