Abstract
FREE TECHNICAL INFORMATION
Agilent Automation Solutions
Technical Poster: Versatile High-Throughput Automated Compound Management System
According to Agilent, integration of true noncontact acoustic liquid dispensing technology within an Agilent BioCel System can enable a new level of high-throughput automation in a drug discovery environment. This new poster describes the collaboration of Agilent Automation Solutions, EDC Biosystems, and Pfizer Animal Health Division to produce a fully automated compound management solution for screening and developmental assay plates. Automation and throughput requirements demand that workflows account for manipulation of samples in a wide variety of plate formats, such as 384 well, 1536 well, and 3456 well. The objective of the described project was to reduce human manual steps while increasing the capacity and turnaround time of laboratory workflow processes. With this project, the authors describe a protocol to generate 450 compound plates (384 to 384 dispense) per run.
Exeter Analytical
Technical Data: CHN Analysis of Heterogeneous Soil, Plant, and Insect Samples
Using an Exeter Analytical Model 440, the SCRI Center for Crop Research accurately and precisely determined the Carbon (C), Hydrogen (H) and Nitrogen (N) content of a wide range of soil, plant, and insect materials using samples sizes of typically 2e20 mg. With SCRI's plant and soil materials, the detector response linearity of the Model 440 proves to be important as composition can vary within a batch of samples. For slowly combusting samples, the Model 440 offers flexible combustion times and oxygen flows. Using a horizontal sample injection system, the Model 440 automatically removes sample residue after each analysis.
More: www.eai1.com
Genevac
Online Resource: Evaporative Sample Preparation for Clinical and Diagnostic Testing
A new online resource from Genevac brings together the latest technological innovations, applications advances, and links to references that detail the current scope of evaporative sample preparation techniques as used in clinical and diagnostic testing. According to Genevac, evaporative sample preparation for diagnostic clinical tests must be performed scrupulously, and visitors to its new online resource can learn how Genevac evaporators and concentration systems are used by clinical biochemists for a number of testing methodologies including vitamin D analysis, vitamin K analysis, drugs of abuse testing, and neonatal screening. Also available in the technology section of the Genevac Web site is further background information to enable users to understand the detail of the various evaporative and concentration techniques used in these applications.
More: www.genevac.com/clinical
Online Resource: ADME-Tox Studies Benefit from Advances in Evaporative Sample Preparation
A new Web page from Genevac brings together the latest technological innovations, applications advances, and links to references that detail how new fast, safe evaporative sample preparation techniques are benefiting metabolism and toxicology studies.
The page offers Web links to a bibliography of scientific publications in peer-review journals, and insight into how Genevac systems are providing fast, safe sample preparation, free from artifact, contamination, or loss, from simple concentration or drying of solvent extracts from tissue samples right through to quantitative screening of workers or athletes for the presence of a drug. Information is also provided detailing how modern evaporation technology is increasing metabolite recovery in ADMET Studies, improving productivity in toxicology screening and delivering advances in metabolite profiling through better sample preparation.
Integra Biosciences
Informative Guide: Ergonomic Pipette Use and Handling
According to Integra, this new guide provides an informed and unbiased introduction to the ergonomic aspects of pipette design, handling, and use. It is available on request.
PBL InterferonSource
Web Site: InterferonSource
According to PBL InterferonSource, its revamped Web site is a highly dependable resource for interferon researchers that offers an interferon research knowledgebase, a scientific forum and e-commerce capability.
More: www.InterferonSource.com
APPLICATION AREAS
Essen BioScience
CellPlayer Cell Migration 96-Well Assay Kit
Essen BioScience's expanding line of kinetic CellPlayer assays now includes this coculture assay for angiogenesis. The CellPlayer Cell Migration 96-well assay offers a precise and reproducible label-free method for monitoring the key biological processes involved in cell migration. This kinetic assay features automated data collection over a period of hours or days and generates data sets that are optimal for studying pharmacological agents and gene silencing technologies. The assay is run on the Essen IncuCyte Live-Cell Imaging System, a compact automated microscope that resides inside a standard tissue culture incubator. IncuCyte software automates the data collection process and provides the subsequent analysis for the cell migration assay, which means cells remain in the controlled environment for the duration of the experiment.
More: www.essenbioscience.com
Exeter Analytical
CE-440 CHN/O/S Elemental Analyzer
According to Exeter Analytical, its CE-440 CHN/O/S Elemental Analyzer is uniquely capable of accurately analyzing the widest range of sample types to determine the percentage of carbon, hydrogen, nitrogen, oxygen, and sulfur. Using a combined combustion technique makes it possible for the CE-440 to analyze any sample type, from volatiles to difficult to combust materials, including nitrides, graphite fibers, ceramics, and even carbides with melting points of over 2000 °C. The fully automated CE440 delivers simultaneous CHN analysis in less than 5 min, and oxygen and sulfur in only 6 min.
More: www.eai1.com
Fluidigm
Single-Cell Expression Data Offers Detailed Molecular Understanding of Embryonic Development
According to Fluidigm, scientists at the Genome Institute of Singapore recently generated significant single-cell expression data crucial for a detailed molecular understanding of mammalian development from fertilization to embryo implantation, a process known as the preimplantation period. The knowledge gained has a direct impact on clinical applications in the areas of regenerative medicine and assisted reproduction. This study is the first of its kind to apply single-cell gene expression analysis of many genes to hundreds of cells in a developmental system.
Using the Fluidigm BioMark microfluidic technology and the mouse preimplantation embryo as a model, the scientists were able to study the expression of 48 genes from individual cells and applied this to analyze over 600 individual cells from the 1-cell to the 64-cell stage of preimplantation development. This high-throughput single-cell research methodology provides the ability to detect dynamic patterns in cellular behavior. Findings of the study resolve some of the arguments pertaining to cellular differentiation events and places fibroblast growth factor signaling as the primary event in the later cell fate decisions.
More: www.fluidigm.com
Access Array System Amplicon Tagging Protocol
A new protocol for Fluidigm's Access Array System enables sequencing of amplicon libraries using GS FLX Titanium Series reagents on the 454 GS FLX sequencing system. The Access Array integrated fluidic circuit (IFC) automatically generates emPCR-ready libraries by simultaneously combining 48 samples and 48 primer sets to produce 48 uniquely barcoded samples per chip for approximately 7 
per sample.
Fluidigm's Access Array IFC, when used with a 454 Titanium sequencer, can capture up to 24 kb of sequence data per sample or 1.15 MB per IFC.
More: www.fluidigm.com
Malvern Instruments
Zetasizer Nano ZS
Using the Zetasizer Nano ZS, researchers at the University of South Florida's (USF) Department of Physics are able to perform temperature-controlled static and dynamic light scattering (DLS) measurements of protein hydration, protein interactions, and aggregation kinetics in vitro to gain a better understanding of protein aggregation phenomena, including protein crystallization and amyloid fibril growth. The Zetasizer Nano ZS has enabled USF to monitor the effect on the hydration of proteins by salt ions in solution, measuring the hydrodynamic radius of proteins down to plus/minus one-tenth the diameter of a water molecule. Neither chaotropic or cosmotropic salt ions affect overall protein hydration up to salt concentrations of 1 M.
USF also is using data gathered by the Zetasizer Nano ZS to develop models for protein fibril formation, and also has had success in using the Zetasizer Nano ZS to study the nucleation of gold nanoparticles. Unlike static light measurements commonly used to detect nucleation events, DLS separates out the background scattering from small molecular species in gold sols. Therefore, DLS was able to detect nucleation at a significantly earlier stage than has been possible with other approaches.
Microsonic Systems
HENDRIX SM100 Ultrasonic Fluid Processor
According to Microsonic Systems, its HENDRIX SM100 can improve fragment-based drug screening quality by resolubilizing precipitated samples back into solution. Recent data shows that by adding the HENDRIX SM100 to the fragment-based drug discovery process, users are able to resolubilize precipitated fragment libraries and consequently recover the originally lost samples. This process both increases the sample concentrations and improves the accuracy of screening results. Using Lateral Ultrasonic Thrust technology to solubilize, thaw, mix, and suspend is proving to be a highly effective tool to increase screening yields.
More: www.microsonics.com
AUTOMATED SYSTEMS
Artel
Multichannel Verification System
The Artel MVS now generates measurements traceable to national and international standards of volumes dispensed to each well in a 384-well plate. Based on ratiometric photometry, MVS users of 96– or 384-well plates are now able to compare liquid handler performance from site to site and from instrument to instrument. The Artel MVS now can evaluate 16– and 24-channel pipettors, and pipettors with 1, 2, 4, 8, 12, 96, and 384 channels; and users of eight-channel dispensers now can se the Artel MVS to perform accurate volume readings with additional dispense pattern options. With an eight-channel plate reader, the Artel MVS now can read a 384-well microtiter plate 4 times faster than before, in just 1 min. The Artel PowerWave, a monochromator-based plate reader, can be used at a wide range of wavelengths, allowing it to operate with multiple applications. Other enhancements include changes to the system's calibrator plate, a new design that allows for more measurements per cuvette in Data Manager version 2.4 and higher, and easier to clean cuvette surfaces. Existing MVS users receive updates to Data Manager at no charge.
More: www.artel-usa.com
Thermo Fisher Scientific
eVol Dispensing System
The new eVol Dispensing System is designed to allow precise and accurate operator-independent dispensing, and is considered ideal for routine dispensing of volatile solvents and hazardous, corrosive, or viscous chemicals. The system is easily calibrated by users to ensure validity of results in full compliance with GLP and good manufacturing practice protocols. Useful for a range of applications—including preparation of calibration standards, addition of internal standards, precise dispensing of nonaqueous liquids, and sample dilution—the eVol offers a simple dispensing solution for laboratories involved with forensics, toxicology, environmental, pharmaceutical, and food safety analysis.
INSTRUMENTATION AND HARDWARE
A2 Technologies
Exoscan or FlexScan Portable FTIR Systems
A2 Technologies now offers a family of portable handheld FTIR systems optimized for geoscience applications, including the compositional analysis of rocks, minerals, and soils. A2s Exoscan and FlexScan FTIR systems are battery powered, handheld FTIR spectrometers that are proven for field applications. With these new portable FTIR systems, samples no longer need to be transported back to the lab. Critical information about a field site can be obtained in real time and can identify minerals and soil components and quantify molecular compounds, including oil contamination or degree of hydration in soil.
The Exoscan system, with its optional Docking Station, also can serve as a benchtop lab system. The lightweight FlexScan is considered an ideal at-site system for analyzing samples over large areas where many measurements are required.
More: www.a2technologies.com
Dorton Analytical
The NQAD Nano Quantity Analyte Detector
According to Dorton, its NQAD Nano Quantity Analyte Detector is a new and completely different kind of aerosol-based detector for HPLC. Based on condensation nucleation (CNLSD) technology, the NQAD can detect subnanogram levels. Benefiting from ultra-high sensitivity, the detector is considered ideal for searching for drug impurities, degradation products, excipients, and when industrial hygiene is a concern. Both previously easy and hard-to-measure compounds can be quantified accurately with minimal sample preparation.
The NQAD detector also offers a wide dynamic range with superior linearity when compared with other aerosol-based detectors. The detection of the NQAD detector is near universal for compounds that are less volatile than the mobile phase. Chromophores and fluorophores are not needed for detection, thereby eliminating or decreasing the need for compound derivatization. The detector is optimized for use in UHPLC, HPLC, and SFC. Easy to install and operate, it has one of the smallest footprints available.
Legato 200 Series Syringe Pumps
According to Dorton, a full-size touch screen interface enables users to quickly create configurations and recall them for easy use while an intuitive run screen combines multiple parameters simultaneously with internationally recognized graphic icons that allow the Legato 200 series to provide a new level of intuitive syringe pump operation. Three basic models—Infuse only, Infuse and Withdraw, and Push Pull—are available in programmable versions for maximum flexibility and capability. Each works with one syringe or two and can be reconfigured in the field to use with multiple syringes.
The Legato Series has a small benchtop footprint (3.5 in. × 9.75 in.), and models can be placed on their sides to reduce the footprint by four times.
FEI Company
Helios NanoLab x50 DualBeam Series
The new Helios NanoLab x50 DualBeam Series is, according to FEI, the most powerful and versatile DualBeam system available on the market today. It integrates FEI's extreme high-resolution scanning electron microscope with a new, high-performance focused ion beam, to deliver an unprecedented level of imaging and milling capability for leading-edge applications in semiconductor and materials science research and development.
More: www.fei.com
Fluid Metering
CTS Chemical Treatment System
This new solution for accurately delivering caustic soda and other pH adjusting chemicals for treatment of water and wastewater uses a unique Valveless Piston Technology. A single rotating and reciprocating ceramic piston accomplishes both the pumping and valving functions, thereby eliminating the need for check valves and the associated maintenance. Sapphire-hard internal ceramics insure that there is no degradation in accuracy over time, and therefore, no need to recalibrate. At a fraction of the size and weight of pumps typically used for caustic soda and soda ash water treatment, Fluid Metering's CTS Chemical Treatment System's space-saving design measures 15 1/2 in. × 13 1/2 in. × 7 in. and weighs only 14 lb and operates on less than 100 W of power. The fluidics are contained in a NEMA 4× protective enclosure that can be wall mounted. More: www.Chloritrol.com
Fluidigm
FC1 Cycler
According to Fluidigm, its new FC1 Cycler can reduce thermal cycling time by a factor of three when compared with the company's current products, and can reduce overall system configuration costs for users conducting high-sample throughput SNP genotyping because fewer thermal cyclers achieve output requirements. Adding the new FC1 Cycler to an existing Fluidigm system can double the number of microfluidic chips processed in a single day. The FC1 Cycler also integrates a self-contained vacuum source and touch screen interface (that offers easy editing of thermal protocols) in a streamlined design that minimizes lab space requirements.
The new FC1 Cycler is fully compatible with Fluidigm's 96.96 and 48.48 Dynamic Array IFCs and its 12.765 Digital Array IFC. The FC1 Cycler, combined with either the Fluidigm EP1 Reader or BioMark real-time polymerase chain reaction system, can complete the entire workflow of the system in a matter of hours with only minutes of hands-on time for loading the chips. This is compared with days of hands-on time and weeks of effort to complete this volume of work using traditional 384-well plates.
More: www.fluidigm.com/pdf/fldm/FLDM_MRKT00145%20FC1%20cycler%20Datasheet.pdf
Integrated DNA Technologies
ZEN Double-Quenched Probes
According to IDT, its new double-quenched probe increases the accuracy and reliability of 5′ nuclease qPCR experiments. Although traditional probes have 20-30 bases between the dye and quencher, this probe design positions an internal ZEN quencher only 9 bases from the 5′ fluorophore. This shortened distance, particularly when combined with the standard 3′ quencher, significantly decreases background fluorescence and increases sensitivity. The chemical structure of the ZEN quencher stabilizes duplex formation, which allows for its use in previously validated sequences. The improved functionality significantly increases qPCR accuracy and sensitivity when compared with traditional probes.
More: www.idtdna.com
Jeio Tech
Lab Companion SKC High Capacity/High Speed Shakers
According to Jeio Tech, because of a newly engineered direct drive mechanism, almost no load is too heavy for the Lab Companion SKC shakers—even at high speeds. A large selection of models is available based on platform size and stroke diameter. Programmable motion control, LED displays, unbalanced load sensors, and self-protecting cut-off function make these shakers versatile and safe. The SKC series offers speeds up to 500 rpm, have a maximum orbit of 2.8 in. (70 mm), and clamps that accommodate up to 6-L flasks. Accessory options allow users to work with a variety of flask sizes, tubes, and bottles. Sticky Pad eliminates flask clamps altogether, whereas Spring Wire Racks and Dual Tiered Platforms add flexibility and capacity.
More: www.jeiotech.com/eng
PANalytical
CubiX3
This third generation range of CubiX X-ray powder diffractometers introduces new features such as high-intensity data collection and extra flexibility in sample handling for automated environments. The CubiX3 range includes dedicated versions, tailored to the needs of those working in cement, minerals, pharmaceuticals, or aluminum production. The Walk-Up software available for CubiX3 enables users without knowledge of XRD to enter urgent samples at any time, even if the diffractometer is already processing a routine batch; and automatically receive a report of results with no user input to the analysis.
Thermo Fisher Scientific
Barnstead TII Water Purification System
This new water purification system produces water that exceeds the American Society of Testing and Materials and the Clinical and Laboratory Standards Institute-Clinical Laboratory Reagent Water type II water standards. With total organic carbon content below 5 ppb, it provides highly pure feed water to the Thermo Scientific Barnstead Nanopure systems and links with the Thermo Scientific Barnstead TII Storage Reservoir. With an exclusive flow pattern that recirculates water between the unit and storage reservoir, degradation as a result of storage is eliminated.
More: http://www.thermo.com/waterbook and www.thermo.com/purewater
Column Oven
Recognizing the importance of temperature as a parameter in liquid chromatography, the new Column Oven delivers exact temperature control to ±0.1 °C coupled with the highest levels of reproducibility and stability. The temperature control unit of Column Oven is designed for simple, fast manual control of the column temperature or alternatively, optional drivers can be used to control the Column Oven through the instrument software.
The Column Oven comes in two specifications. Column Oven 200 can accommodate up to 4 analytical HPLC columns with lengths up to 150 mm. Column Oven 300 can contain as many as 4 analytical columns with lengths to 250 mm. Either configuration can be mounted on a CTC autosampler or many Thermo Scientific mass spectrometer ion sources.
TouchMark
Printing with Radiopaque Inks
Touchmark has a printing process for medical applications using radiopaque inks. Radiopaque ink is considered ideal for printing visible tags used with X-Ray, MRI, or other imaging equipment. The ink is easily seen on film or using a fluoroscope. TouchMark's specialized process provides permanent ink adhesion on virtually any surface or substrate without flaking or chipping. TouchMark's expertise includes printing on difficult surfaces such as FEP and Nitinol.
More: www.padprint.com
Zinsser Analytic
Digilab CellJet Cell Printer
The CellJet Cell Printer incorporates Digilab's synQUAD liquid dispensing technology, offering both on-the-fly and drop-by-drop noncontact cell printing while maintaining the viability of even the most delicate cells. According to Zinsser, this flexible technology offers full control over critical dispense parameters such as height of dispense and dispensing speed, which allows printing of both viscous solutions and fragile cells. The Digilab CellJet prints into plates or onto slides using a valve-free fluid path, which greatly reduces cell damage. The printer also includes full wash capability, an easy-to-clean and sterilize evaporation cover, and an effective instrument design for hassle-free maintenance. User-friendly software makes it easy to program printing protocols.
More: www.zinsser-analytic.com
LABWARE AND LAB SUPPLIES
Air Science USA
Purair 5 Ductless Fume Hoods
These new units exceed OSHA, ANSI, and all relevant international standards, and the ductless design eliminates installation costs and allows the unit to be positioned over a sink or benchtop apparatus.
Three models offer a choice of 24, 36, or 48 in. widths that operate at low noise levels. Because they recirculate, they do not exhaust conditioned and/or heated air into the atmosphere. A face velocity of 100 fpm ensures containment of fumes and particulates for operator protection. An alarm alerts the operator when the airflow falls to an unacceptable level.
More: www.air-science.com
AMS Biotechnology
Turbo Dicer siRNA Generation Kit
According to AMS Biotechnology (AMSBIO), its new Turbo Dicer siRNA Generation Kit enables easy and cost-effective generation of a large number of small interfering RNAs (siRNAs) from full-length target genes. siRNAs are 21– to 23-nucleotide RNA molecules that can cause targeted gene silencing in mammalian cells through RNA interference.
Using an ultra-active form of human recombinant dicer enzyme, a double-stranded RNA-specific endonuclease, it is possible using the kit to cleave more than 95% of dsRNA template into 22 bp siRNAs within 2 h under optimized reaction conditions. In contrast to individual synthetic siRNAs, the Turbo Dicer kit allows quick production of multiple siRNA species against target mRNAs to achieve effective gene silencing with minimal guesswork. Because minimal concentration of any individual siRNA species is generated with the Turbo Dicer Enzyme, off-target effects are not a problem.
MAPTrix ECM Nonanimal Recombinant Proteins
The Mussel Adhesive Protein based matrix (MAPTrix) ECM line of products are coating reagents with genetically incorporated bioactive peptides that mimic the extracellular matrix (ECM) activity of collagen, fibronectin, laminin, and vitronectin proteins. These bioactive peptides mimic ECM proteins and provide for cell attachment, spreading, and growth. A MAPTrix surface coating offers an animal-free, chemically defined attachment and growth surface that provides a highly controlled environment for cell culture and tissue engineering applications. MAPTrix ECM products can be used for cell culture application alone or in combination with other products. All MAPTrix products are soluble in a variety of buffers including water under a wide range of pH (pH = 2.0-9.5). Available for basic research or drug discovery applications in serum-free or serum-reduced conditions, MAPTrix products are ready-to-use aqueous coating formulations in 1.0, 2.5, 5.0, and 10.0 mg sizes (vials).
Tissue Products for Genomics and Proteomics
AMSBIO's new comprehensive range of certified tissue products for genomic and proteomic research includes individual genomic DNA preparations, together with 96-well gDNA plates from tumor and normal tissue for Epigenetics studies. Tissue-specific polymerase chain reaction (PCR)-ready first strand cDNAs, universal cDNA, and cDNA panels are available for Gene expression studies. AMSBIO total and universal RNA products are isolated with microRNAs and are available with RIN values, on request, for Microarray work. Total protein products from normal and disease state tissues add further research possibilities with whole tissue lysates and subcellular fractions from the nucleus, cytoplasm, cell membrane, and cytoskeleton.
Beckman Coulter
Solastra Five-Color Reagent Panel
Solastra is a comprehensive new CE-marked panel of conjugated-antibody cocktails for the characterization of hematolymphoid neoplasia by flow cytometry. These premixed and ready-to-use panels target the appropriate markers as recommended by the 2006 Bethesda International Consensus and are compatible with the WHO Classification of Tumors of Hæmatopoietic and Lymphoid Tissues. Twenty-three markers are combined in seven tubes—two tubes in the B-cell lineage kit, two tubes in the T-cell lineage kit, and three tubes in the myelomonocytic lineage kit—each tube containing up to five relevant antibodies. Kits can be run individually or jointly.
In addition to CD45, which is included in all seven tubes, the markers represented in the B-cell lineage kit are CD5, CD10, CD19, CD20, CD38, Kappa, and Lambda. Tubes in the T-cell lineage kit offer CD2, CD3, CD4, CD5, CD7, CD8, and CD56. The myelomonocytic lineage kit tubes contain CD7, CD11b, CD13, CD14, CD15, CD16, CD33, CD34, CD56, CD117, and HLA-DR. Each antibody is conjugated to one of five dyes: FITC, PE, ECD (PE-Texas Red), PC5.5 (PE-Cyanine 5.5), and PC7 (PE-Cyanine 7).
More: www.beckmancoulter.com
Cell Lab Signaling Cytometry
The Cell Lab SC line of reagents from Beckman Coulter offers a comprehensive set of antibodies for the detection of the phosphorylation state of signaling proteins in cells via flow cytometry. Performing cell-signaling experiments via flow enables the user to generate precise, specific, and quantitative measurements of the signaling response in individual cellular subpopulations. The reagents, which in the initial offering are conjugated to either Alexa Fluor 488 or Alexa Fluor 647, are released in conjunction with a new method that allows analysis of samples in whole blood. Analyzing samples in whole blood minimizes artifacts and variability introduced through sample manipulation and helps preserve the native biology of the cell. According to Beckman, the method has also shown excellent performance when used with bone marrow, cell culture, and purified cell populations.
Antibodies in the Cell Lab SC line can be used in combination with extracellular markers to study signaling patterns in specific cellular subpopulations. Products are available for the study of apoptosis, chromatin regulation, cytoskeletal signaling, DNA damage, lymphocyte signaling, MAP kinase signaling, protein folding, translation control, and other phosphorylation events.
More: www.beckmancoulter.com
BioFocus
SoftFocus Kinase Libraries
BioFocus' three new SoftFocus kinase libraries (SFK61, SFK62, and SFK63) target the hinge region, allosteric sites, or novel binding modes. According to BioFocus, its new SFK collection has been independently determined to have the greatest population of kinase-like molecules available for screening.
Helix Mimetic Library
BioFocus' second Helix Mimetic library (HM02) is based on a novel structure that interacts with protein helices. This library is designed to inhibit protein—protein interactions, but can also target other helical recognition motifs such as the pore region of ion channels.
Biohit
mLINE PCR Starter Kit
Biohit's new mLINE polymerase chain reaction (PCR) Starter Kit for PCR applications includes three pipettes covering the standard volumes used in PCR along with sterile and certified SafetySpace filter tips that guarantee contamination free and safe pipetting. In addition, the kit includes a pipette holder, color-coded caps, microtube opener/calibration tool, cooling rack, cryo pen, and instructive literature. The mLINE PCR Starter Kit offers light and accurate manual pipetting. The fully autoclavable mLINE pipettes and the DNase and RNAse certified and sterile filter tips ensure clean PCR processes.
More: www.biohit.com
Hamilton Company
GASTIGHT HPLC Syringes
This new line of rugged syringes is designed for optimal performance and long life in Spark Holland HPLC Autosamplers. These new GASTIGHT syringes are considered ideal for dispensing liquid samples from a wide range of matrices and solvents. The polytetrafluoroethylene construction of the syringe plunger tip ensures a precise fit with the glass barrel, creating a leak-free seal for accurate operation. Hamilton GASTIGHT models are well suited for use with heterogeneous samples.
More: www.hamiltoncompany.com
INTEGRA Biosciences/Viaflo Corporation
GripTips
This next-generation range of pipette tips is designed to ensure a perfect connection between pipette and tip. GripTips are engineered for unmatched high-lateral resistance. As a result, pipette tips will never fall off while pipetting. Benefiting from unique Tri-Lobe Fittings, GripTips deliver low attachment and ejection forces enabling comfortable, stress-free pipetting even over extended periods of use. GripTips deliver a precise and consistent tip seal ensuring that all tips on a multichannel pipette are precisely at the same height; increasing the accuracy and precision of each multichannel dispense.
More: www.viaflow.com
Micronic Europe
DNA-Free Consumables for Forensic Sample Storage
Micronic Europe offers a range of new storage tubes (0.50, 0.75, 1.10, and 1.40 mL) and capping products that absolutely secure the long-term integrity of DNA samples for forensic analysis.
Using a new ethylene oxide treatment process, this new range of screw top and push cap tubes are independently certified to be absolutely DNA free. Manufactured from medical grade polypropylene, in a fully automated class 7 clean room environment, these tubes exhibit absolute product consistency and near zero contaminants. A unique 2D code on the bottom of each tube provides an easy and unambiguous means of identifying samples. The optimized internal shape of each tube ensures the lowest possible dead volume and maximum sample recovery. Designed to fit 96 individual tubes into a standard footprint rack optimizes the use of valuable freezer space and ensures automation compatibility.
More: www.micronic.com
QIAGEN
Investigator PCR Assays
According to Qiagen, its new portfolio of 12 Investigator polymerase chain reaction (PCR) assays for human identification features the most comprehensive and versatile coverage of current STR standards, including the new extended European Standard Set (ESS), CODIS, DAD, and ISSOL requirements. The new portfolio is designed to complement QIAGEN's extraction and sample management solutions, and address the Council of the European Union resolution on the exchange of national DNA database information. To facilitate and standardize this exchange, the ESS of STR markers is expanded.
More: www.qiagen.com/forensics
miScript Target Protector
This product specifically interferes with the interaction of an miRNA with a single target, while leaving the regulation of other targets of the same miRNA unaffected. With this approach, transfection of an miScript Target Protector can reveal the targets and roles of specific miRNAs.
More: www.qiagen.com
FlexiTube siRNA Premix
A new tool for functional genomics and drug discovery research, siRNA and a transfection reagent are premixed at optimized ratios. This provides the benefits of potent siRNA design and efficient transfection, while minimizing the need for tedious optimization, shortening the transfection protocol and enabling consistency across experiments and more reliable results.
More: www.qiagen.com
PyroMark CpG Assays
These pyrosequencing assays enable high-resolution methylation analysis of specific targets across the genome. Using pyrosequencing, consecutive methylation sites can be analyzed independently and quantitative methylation data can be obtained in a fast and convenient way. New PyroMark CpG Assays are predesigned pyrosequencing assays and eliminate the time-consuming and error-prone assay design process in epigenetics research.
More: www.qiagen.com
PyroMark Custom Assays
These new assays are customer designed and can be used for any type of pyrosequencing research application: epigenetics; genotyping; microbial ID, and resistance typing. By designating PCR and sequencing primers, users can generate optimized, user-specific assays to determine methylation patterns, the frequency of insertions, and deletions or other mutations or to obtain sequence information of virtually any gene.
More: www.qiagen.com
PyroMark OneStep RT-PCR Kit
This is a new one-step RT-PCR kit for the identification and resistance typing of RNA viruses using pyrosequencing technology. It generates a high-quality sequencing template in the quantities needed for sensitive mutation and microbial analysis.
More: www.qiagen.com
Thermo Fisher Scientific
Jewett Laboratory Refrigerator and Freezer Range
Specifically designed to store high value, critical samples, reagents, vaccines, blood, and plasma, the new high-performance Jewett collection incorporates 24 models ranging from the 4.9 ft3 under counter models, through to 51.1 ft3 upright models, providing users with a unit for a variety of blood bank, plasma, pharmacy, and general applications. The Jewett product family also offers features specially designed blood bank and plasma storage such as built-in chart recorders, locking self-closing doors, and sliding stainless steel drawers.
The new models include microprocessor control for precise temperature regulation and set-point security, enabling users to program and lock set temperatures to prevent unauthorized adjustments. The range also is easy to use with adjustable shelves that are easily removed for effective cleaning, and an ergonomic handle and self-closing doors for ease and efficiency when accessing samples. Other features include powerful industrial grade compressors and positive, forced-air circulation, which together ensure a rapid return to the set temperature even with frequent freezer access. All models are equipped with visual and audible alarms, backed up by battery, to alert the user of any fall in temperature below desired setting.
Hypersil GOLD HPLC Columns
According to Thermo, Hypersil GOLD high-performance liquid chromatography (HPLC) columns are now available in 11 phases, making the Hypersil GOLD family one of the most comprehensive ranges available. In addition to a C4 phase that broadens the scope of selectivities for reversed phase LC, new columns also include options for the analysis of polar compounds by ion exchange, HILIC, and normal phase chromatography. All phases use the same backbone of pure silica to deliver outstandingly symmetrical peak shapes, regardless of the compound or mode of analysis.
Hypersil GOLD C4 is a low hydrophobicity column with short alkyl chain length. This has the effect of shortening retention, making the column ideal for reversed phase separations of analytes such as proteins and peptides. Hypersil GOLD amino columns are considered well suited to the analysis of sugars and carbohydrates, demonstrating excellent properties in three modes: weak anion exchange, reversed, and normal phases. Hypersil GOLD AX is considered ideal for the separation of proteins, peptides, other anionic species, and polar molecules, and can be used as both an anion exchange column and for HILIC separations. As a highly stable quaternary amine strong anion exchange column, designed for aqueous mobile phase, the Hypersil GOLD SAX is considered ideally suited for use with smaller organic molecules, including nucleotides and organic acids. The final addition to the portfolio, Hypersil GOLD Silica is considered a powerful and efficient tool for the analysis of nonpolar and moderately polar organic compounds using normal phase LC.
MICROPLATES
Porvair Sciences
Black Microplate and Seal Combination
Manufactured from polypropylene, Porvair's black 96-deep well microplate offers excellent heat and solvent resistant qualities. Using only ultra-pure grade polymer means that each black plate has near zero leachates ensuring long-term sample integrity. Porvair black plates are supplied RNase/DNase free so they can be used with even the most sensitive biological samples. Offering a sample volume of 1 mL/well with round well bottoms for good mixing, Porvair black plates are precisely manufactured to applicable ANSI/SBS dimensions ensuring complete compatibility with almost all readers and automated equipment.
AbsorbMax sealing films are 200-μm thick black vinyl adhesive sealing films for use with 96-well black microplates. A split backing aids in applying the sealing film properly. Edge cutouts assist in positioning and accommodate ribs on the plate bottom. The light-blocking and absorbing properties of AbsorbMax sealing films are considered ideal, in combination with Porvair black 96-well plates, for protecting light-sensitive assays or samples during storage.
More: www.porvair-sciences.com
Acrylic Manifold
Precision machined from crystal clear acrylic (top plate) and acetal polymer (plenum chamber), this vacuum manifold allows a collection plate to be viewed throughout the entire elution process to monitor progress of the separation process. Designed to be fully compatible with all ANSI/SBS standard compliant solid-phase extraction, filtration, protein precipitation, and collection plates, the acrylic manifold is fitted with a valve controller to ensure accurate adjustments of vacuum to the manifold, and a custom upper surface O-ring provides an airtight interface between plates during operation. As a consequence plates (up to 45 mm in height) may be processed with high reproducibility. The acrylic manifold is supplied with an adapter to fit standard 350, 1, and 2 mL/well plates, a waste collection reservoir tray, a square well collection plate, and a set of simple operating instructions.
More: www.porvair-sciences.com
Aluminum Heat Seals for PCR, Storage, and Life Science Applications
According to Porvair, these new foils are precision engineered to an exact thickness and come color coded for easy identification. The precut foil sheets are marked to ensure that they are always used the right way up, thus minimizing the possible damage to the sealing head of a thermal heat sealer that can occur when a foil is inserted the wrong way.
Four different foils are available: 229480 is a 20 micron foil, color-coded green, designed for very easy piercing by robotic liquid handling or pipette tips; 229471 is a resealable 38 micron foil, color-coded blue, that is easily pierced and peeled; 229470 is a 70 micron nonpiercable foil, color-coded red, that offers good tear strength and has an easily pealed polyester backing; and 229482 is an 85 micron foil, color-coded black, for heavy duty use, including storage and transport applications.
More: www.porvair-sciences.com
INFORMATICS AND COMPUTING
Agilent Automation Solutions
VWorks Automation Control Software
According to Agilent, its VWorks Software offers integration of a wide range of devices such as robots, liquid handlers, readers, imagers, bulk reagent dispensers, microplate handlers, shakers, stores and washers, thermal cyclers, sealers, piercers, and others to deliver a cohesive, integrated system that ensures maximum throughput and optimal resource utilization. An intuitive graphical user interface makes it easier for users to create new protocols, connect and configure devices, run protocols, and monitor progress. The latest version of VWorks offers full 21CFR Part11 compliance support, making it an optimal solution for applications covered in FDA guidelines.
More: www.chem.agilent.com/en-us/products/instruments/automation/software/vworks/pages/default.aspx
G.E. Healthcare
DeCyder 2D 7.1 SPN Software
Specifically designed to support 2-D DIGE for the characterization and quantitation of host cell proteins (HCPs), this new software package offers an antibody-independent procedure to enable direct comparisons of individual target protein/HCP profiles between samples taken from distinctly different steps of a process. This capability increases process understanding, and facilitates process optimization and Quality by Design.
More: www.gelifesciences.com
Nuix
Collector for SharePoint
According to Nuix, its new Collector for SharePoint is the world's only forensically defensible means of collecting all Electronically Stored Information (ESI) from Microsoft SharePoint sites, including dynamic content. It can collect discussion threads, blogs, wikis, calendars, task lists, and all associated data in a fully automated fashion, and allows it to be rendered via the SharePoint site.
Nuix Collector for SharePoint supports Microsoft SharePoint WSS, 2007 and 2010, and cloud-based SharePoint sites. Nuix Collector is a key part of a powerful, simple to use and forensically sound suite of tools for the identification, collection, and preservation of potentially relevant ESI.
More: www.nuix.com
Olympus
cellSens Microscope Imaging Software
According to Olympus, this comprehensive, fully customizable solution allows users to move smoothly and quickly from initial image capture through processing, analysis, and reporting in just a few simple steps. Even such advanced functions as deconvolution, cell counting, large area stitching, fluorescence unmixing, and data sharing are fast and intuitive. The customizable SQL Server Express-based database can be used locally or across global networks. The Olympus NetCam module offers live viewing over the Internet. Users can exchange images and view data offline using cellSens Viewer software, which is available for free. Users can further explore resulting images with advanced 3D visualization via the software's Voxel-Viewer feature.
PANalytical
Omnian and MiniPal 4
PANalytical has extended the compatibility of its Omnian advanced standardless analysis software to include the compact benchtop MiniPal 4 X-ray fluorescence (XRF) spectrometer. According to the company, this combination offers a premium XRF solution that is easy to use, powerful, and considered ideal for routine lab and field analysis, offering quantification of unknowns in situations where certified standards are not available.
More: www.panalytical.com
SP Scientific
FTS SMART Freeze Dryer Technology
Designed to accelerate and streamline the development and optimization of freeze-drying cycles, the FTS SMART Freeze Dryer control software tool is based on unique control algorithms developed by lyophilization experts and is now available exclusively for the SP Scientific LyoStar and Process Development freeze dryer ranges. SMART Freeze Dryer technology allows users to create streamlined but safe cycles that can shave days off production cycles, and features a unique feedback loop that provides detailed data on the pharmaceutical product and the freeze-drying process, including product resistance, heat flow, and product thickness.
More: www.spscientific.com
Thermo Fischer Scientific
SkanIt Software Version 3.0
For the Multiskan FC microplate photometer, this new version of the SkanIt Software provides eight inbuilt languages: English, French, German, Spanish, Portuguese, Chinese, Japanese, and Russian. All the languages are available after software installation and can be changed with ease. Version 3.0 also incorporates the parallel line analysis function in compliance with European Union regulatory guidelines (European Pharmacopoeia) for testing the efficacy of pharmaceutical compounds. With ready-made sessions downloadable from www.thermoscientific.com/readingroom, the user-friendly interface facilitates simple assay set-up for both instrument control and data handling. The software is designed with a variety of inbuilt calculations, including blank subtraction, curve fits, and basic statistics (Avg, CV%, SD). Measurement results can be analyzed or exported to Microsoft Excel.
Titian Software
Mosaic Sample Management Software Version 4.0
Applications of the new Mosaic 4.0 are extended to the enterprise-wide management of biological samples and reagents in many different types of life science organizations including industry and academia. Mosaic 4.0 includes modular scalability, advanced instrument integration, and comprehensive audit trail and genealogy tracking. For the specific requirements of biological sample management, Mosaic 4.0 now incorporates enhanced modules for tracking manually accessed stores and manual operations, such as hand pipetting for smaller-scale operations. A new built-in Substance Registration System handles the allocation of substance identifiers and the capture of substance metadata, and Mosaic can seamlessly link to external registration systems if required. For higher-throughput laboratories, Mosaic 4.0 builds on its workflow management capability and wide array of integration modules for automated stores, liquid handlers, and other instruments. The new functionality is considered well suited to the management of clinical trial samples and reagents, therapeutic and antidrug antibodies, cell lines, and biobank samples—in addition to small molecule compounds.
More: www.titian.co.uk
Ziath
DataPaq Version 1.23
A new version of Ziath's 2D barcode scanning software is now available as a free upgrade to existing users. The new version delivers improved empty well detection with zero-configuration that works out of the box for all tube types; improved ability to detect when a plate is not on the scanner or is unreadable (e.g., when the tubes in the rack are not barcoded); ability to export results to a database by direct connection to database; XML result output to file; keyword configuration for output files; date, time, and barcode replacement in filename for driverless integration mode; retrieval of settings in integration mode; and automatic settings refresh while running in integration mode.
DataPaq Single Version 1.2 also offers new features, including a server mode to facilitate integration into robotics in LIMS systems; configurable rescan of same barcode; and faster performance.
More: www.ziath.com
SERVICES
The Schmersal Group
Safety Consulting
The Schmersal Group's new Safety Consulting service offers comprehensive manufacturer-and technology-neutral consultancy regarding issues such as the implementation of directives and standards in actual constructional practice. The key task of the Schmersal Safety Consultants is providing qualified answers to questions, for example, which type of safety system must be used, how the safety system must be integrated in the control system and the functionalities of the machine, and how a programmable electronic safety system can be configured to optimally meet both the safety requirements and the productivity specifications of a machine. The assessment of the required Performance Level to EN ISO 13849-1 and the calculation of the actual performance level for the selected safety system are two other tasks that the Safety Consultants can accomplish.
More: www.schmersal.com