To clarify the role of viral infection in otitis media, we intranasally inoculated mice with influenza A virus and examined histologic changes in the nasopharyngeal mucosa using a battery of lectins. Additionally, live Haemophilus influenzae o r Streptococcus pneumoniae was injected into the nasopharynx after virus inoculation, and the clearance of bacteria from the nasopharynx was examined. Staining of the mucous blanket and epithelial cell surfaces in the nasopharynx with peanut agglutinin, succinyl wheat-germ agglutinin, and Bandeiraea simplicifolia agglutinin was significantly enhanced with intranasal virus inoculation when compared with that in control animals. The nasopharynx was moderately stained with Maachia amurensis agglutinin and wheat-germ agglutinin in control animals, and the staining was enhanced after virus inoculation. These findings were most remarkable 5 and 9 days after virus inoculation. The numbers of bacteria cultured from the nasopharynx were significantly increased when bacteria were injected 5 days after virus inoculation. These results suggest that an alteration in the glycoconjugate structure lining the nasopharyngeal mucosa caused by the influenza virus might be associated with the reduction in bacterial clearance. (Otolaryngol Head Neck Surgery 1999;121:616–21.)
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References
1.
KuronoYTomonagaKMogiG.Staphylococcus epidermis and Staphylococcus aureus in otitis media with effusion. Arch Otolaryngol Head Neck Surg1988; 114:1262–5.
2.
BernsteinJMFadenHFDryjaDMMicro-ecology of the nasopharyngeal bacterial flora in otitis-prone and non-otitisprone children. Acta Otolaryngol (Stockh)1993; 113:88–92.
3.
KuronoYMogiG.Otitis media with effusion and the nasopharynx. Acta Otolaryngol (Stockh) Suppl1988; 454:214–7.
4.
FainsteinVMusherDMCateTR.Bacterial adherence to pharyngeal cells during viral infection. J Infect Dis1980; 141:172–6.
5.
MeyerhoffWLGiebinkGSSheaDAEffect of tympanostomy tubes on the pathogenesis of acute otitis media. Am J Otolaryngol1982; 3:189–95.
6.
SuzukiKBakaletzLO.Synergistic effect of adenovirus type 1 and nontypeable Haemophilus influenzae in a chinchilla model of experimental otitis media. Infect Immun1994; 62:1710–8.
7.
SharonN.Bacterial lectins, cell-cell recognition and infectious disease. FEBS Lett1987; 217:145–57.
8.
UenoKHanamureYOhyamaM.Differences in terminal carbohydrate structures of sialomucin in the murine nasal cavity. Eur Arch Otorhinolaryngol1994; 251:119–22.
9.
UenoKOhyamaMLimDJ.Expression of sialic acids in the developing murine tubotympanum. Acta Otolaryngol (Stockh)1992; 112:824–30.
10.
UenoKLimDJ.Heterogeneity of glycoconjugates in the secretory cells of the chinchilla middle ear and eustachian tubal epithelia: a lectin-gold cytochemical study. J Histochem Cytochem1991; 39:71–80.
11.
LinderTELimDJDeMariaTF.Changes in the structure of the cell surface carbohydrates of the chinchilla tubotympanum following Streptococcus pneumoniae-induced otitis media. Microb Pathog1992; 13:293–303.
12.
LaMarcoKLDivenWFGlewRH.Experimental alteration of chinchilla middle ear mucosae by bacterial neuraminidase. Ann Otol Rhinol Laryngol1986; 95:304–8.
13.
CarrollSMHigaHHPaulsonJC.Different cell-surface receptor determinants of antigenically similar influenza virus hemagglutinins. J Biol Chem1981; 256:8357–63.
14.
HeikkinenTRuuskanenOWarisMInfluenza vaccination in the prevention of acute otitis media in children. Am J Dis Childhood1991; 145:445–8.
15.
NahoriMAEtievantMTerzidis-TrabelsiHBinding of serum autoantibodies to sialidase-treated tracheal epithelial cells. Determination of autoantibodies isotypes in normal and influenza virus infected guinea pig sera. Immunol Lett1994; 42:67–73.
16.
PaulsonJCWeinsteinJDorlandLNewcastle disease virus contains a linkage specific glycoprotein sialidase. Application to the localization of sialic acid residues in N-linked oligosaccharides of alpha 1-acid glycoprotein. J Biol Chem1994; 257:12734–8.
17.
ChungMHGriffithSRParkKHCytological and histological changes in the middle ear after inoculation of influenza A virus. Acta Otolaryngol1993; 113:81–7.
18.
MasonCMAziziSQDal NogareAR.Respiratory epithelial levels of rats with gram-negative bacillary colonization. J Lab Clin Med1992; 120:740–5.
19.
PlotkowskiMCPuchelleEBeckGAdherence of type I Streptococcus pneumoniae to tracheal epithelium of mice infected with influenza A/PR8 virus. Am Rev Respir Dis1986; 134:1040–4.
20.
St. GemeJWIII.The HMW1 adhesin of nontypeable Haemophilus influenzae recognizes sialylated glycoprotein receptors on cultured human epithelial cells. Infect Immun1994; 62:3881–9.
21.
AndersonBDahmenJFrejdTIdentification of an active disaccharide unit of a glycoconjugate receptor for pneumococci attaching to human pharyngeal epithelial cells. J Exp Med1983; 158:559–70.
22.
ReddyMSMurphyTFFadenHSMiddle ear mucin glycoprotein: purification and interaction with nontypable Haemophilus influenzae and Moraxella catarrhalis. Otolaryngol Head Neck Surg1997; 116:175–80.
