This randomized, double-blind, prospective study aimed to evaluate the clinical effects of different ozone concentrations in a mixture of 98% oxygen and 2% ozone. The dose of this mixture for the so called ozone therapy is calculated using the equation: dose = concentration×volume of the ozone mixture. Our study aimed to evaluate the medical effects on volunteers. Group A received rectal insufflation of 20 μg/ml of the ozone–oxygen mixture in a volume of 39 ml, resulting in a final dose of 780 μg. Group B received 39 μg/ml of the mixture in a volume of 20 ml, also resulting in a final dose of 780 μg. There were 9 volunteers in Group A and 10 in Group B. BDORT measurments were taken just prior and immediately after the rectal ozone insufflation at the voluteer's thymus chest accupuncture point located using thymus hystologic slides. Bi-Digital O- Ring test was used to determine levels of polymerase chain reaction (PCR) an indication of inflammation, mercury as well as Candida albicans. The physician that performed BDORT measurments for each subject's group was not aware of which group the patient belonged to. For Group A, the mean PCR level before rectal insufflation was 1,789 ng, which dropped to 656 ng after the procedure. Mercury levels showed a mean value of 411 mg before and 212 mg after. Candida albicans levels were 1230 ng before and 688 ng after. All parameters demonstrated significant improvement (p < 0.05). For Group B, the mean PCR level before insufflation was 3,604 ng, which decreased to 1,864 ng postprocedure. Mercury levels dropped from 756 ng to 366 mg, and Candida albicans levels decreased from 1,641 ng to 957 ng. Group B also showed significant improvement (p < 0.05). All values represent mean values. When comparing the mean values, PCR decreased by 1,133 ng in Group A and 1,740 ng in Group B. Mercury levels decreased by 199 mg in Group A and 390 mg in Group B. Candida albicans levels decreased by 542 ng in Group A and 684 ng in Group B. These findings suggest that the concentration of 39 μg/ml in a volume of 20 ml showed better clinical outcomes than the concentration of 20 μg/ml in a volume of 39 ml, as evaluated using BDORT slides and technique.

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Ozone therapy: Which concentration shows better clinical results? a comparison between 20 μg/ml and 39 μg/ml rectal insufflation—a prospective,double-blind,randomized study